Farzami Marjan Rahnamaye, Aliasgharpour Mehri
Department of Biochemistry, Reference Health Laboratory, Ministry of Health & Medical Education, Tehran, Iran.
J Diabetes Metab Disord. 2017 Jun 5;16:24. doi: 10.1186/s40200-017-0305-7. eCollection 2017.
Human prolactin (PRL) is a hormone that is mainly secreted by lactotroph cells of the anterior pituitary gland and is involved in many biological processes including lactation and reproduction. Prolactin level may be determined quantitatively in serum by many different systems including chemiluminescence systems. However, comparison of the measured values between systems is difficult.
The prolactin values obtained and compared in two chemiluminescence systems; AUTOBIO DIAGNOSTICS MICROPLATE LUMOMETER and LIAISON XL Analyzer using BioRad tri level serum quality control materials and serum samples from = 44 female patients; (Age mean & range) = (33: 21-65) years.
Obtained PRL mean and range in Autobio and Liason systems were X = 414.8 ± 230.0; Range: 25.7-980.9 μlU/mL & X = 391.7 ± 225.6; Range: 26.0-991.4 μlU/mL respectively. Both system's results were correlated (Pearson product moment correlation = 0.97 at = 0.01 and Regression Analysis).
Because of the differences between CLIA systems the authors conclude laboratories measuring range for PRL be accomplished on particular analyzer and verified against reference intervals stated by the manufacture. More importantly, consecutive PRL level determinations and patients follow up should be performed on only one analyzer rather than different analyzers. In this regard, mentioning the method and system type on the final laboratory reports become important and verify that a laboratory considers the clinical aspects of laboratory request as well as the quality assurance in performing the PRL determination.
人催乳素（PRL）是一种主要由垂体前叶催乳细胞分泌的激素，参与包括泌乳和生殖在内的许多生物学过程。催乳素水平可通过包括化学发光系统在内的许多不同系统在血清中进行定量测定。然而，不同系统之间测量值的比较很困难。
使用BioRad三级血清质量控制材料和来自44名女性患者（年龄均值及范围 = 33: 21 - 65岁）的血清样本，在两种化学发光系统（AUTOBIO DIAGNOSTICS微孔板光度计和LIAISON XL分析仪）中获得并比较催乳素值。
在Autobio和Liason系统中获得的PRL均值及范围分别为X = 414.8 ± 230.0；范围：25.7 - 980.9 μlU/mL和X = 391.7 ± 225.6；范围：26.0 - 991.4 μlU/mL。两个系统的结果具有相关性（在α = 0.01时，Pearson积矩相关性 = 0.97，进行回归分析）。
由于化学发光免疫分析（CLIA）系统之间存在差异，作者得出结论，测定PRL的实验室测量范围应在特定分析仪上完成，并根据制造商规定的参考区间进行验证。更重要的是，连续的PRL水平测定和患者随访应仅在一台分析仪上进行，而不是在不同的分析仪上进行。在这方面，在最终实验室报告中提及方法和系统类型变得很重要，并验证实验室在进行PRL测定时既考虑了实验室请求的临床方面，也考虑了质量保证。
