Protective effect of extract on -methyl-nitrosourea (NMU) induced prostate cancer in rats.

BACKGROUND

The main objective was to evaluate the possible protective effect of extract on -methyl nitrosourea (NMU)-induced prostate cancer in rats and DU-145 cell line.

MATERIALS AND METHODS

Prostate carcinogenesis was induced in 30 male Holtzman rats by providing cyproterone acetate, testosterone, and NMU. The tumors were monitored and hematological and biochemical parameters and frequency of micronucleated polychromatic erythrocytes were recorded. The cell line was assessed by a cytotoxicity assay.

RESULTS

Oral administration of extract significantly lowered superoxide dismutase malondialdehyde, NO, C-reactive protein, and prostate-specific antigen levels (all  < 0.01 compared with Inductor Group). There was a significant decrease in the frequency of micronucleated polychromatic erythrocytes ( < 0.05). presented a selectivity index of 17.24 in the cytotoxicity assay.

CONCLUSIONS

Considering its anti-inflammatory, antioxidant, and antigenotoxic effects, and important variations on biochemical and hematological parameters, including prostate-specific antigen of extract, we conclude that it has a protective effect on NMU-induced prostate cancer in rats and cytotoxicity in the DU-145 cell line.