抗L-脯氨酰-亮氨酰-甘氨酰胺血清对催产素的免疫化学识别。

Immunochemical recognition of oxytocin by antiserum to L-prolyl-leucyl-glycine amide.

作者信息

Watkins W B, Peek J C

出版信息

Neurosci Lett. 1985 Mar 15;54(2-3):283-8. doi: 10.1016/s0304-3940(85)80092-6.

Abstract

Antisera were raised in rabbits against Pro-Leu-Gly-NH2 (PLG)-bovine thyroglobulin conjugates. Specificity of the antisera towards oxytocin was evaluated by a radioimmunoassay as well as by immunohistochemical procedures on hypothalamic tissue and hormone bound to Sepharose beads. It was concluded that immunization with PLG can raise antisera that are specific to oxytocin.

摘要

用抗Pro-Leu-Gly-NH2（PLG）-牛甲状腺球蛋白偶联物在兔体内制备抗血清。通过放射免疫测定以及对下丘脑组织和结合到琼脂糖珠上的激素进行免疫组织化学程序，评估抗血清对催产素的特异性。得出的结论是，用PLG免疫可产生对催产素特异的抗血清。