引入乙酰辅酶 A 羧化酶旁路途径到大肠杆菌以提高游离脂肪酸产量。
Introduction of an acetyl-CoA carboxylation bypass into Escherichia coli for enhanced free fatty acid production.
机构信息
School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.
School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea; School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.
出版信息
Bioresour Technol. 2017 Dec;245(Pt B):1627-1633. doi: 10.1016/j.biortech.2017.05.169. Epub 2017 May 31.
This study investigated the effect of the methylmalonyl-CoA carboxyltransferase (MMC) of Propionibacterium freudenreichii on production of free fatty acid (FFA) in Escherichia coli. Overexpression of the MMC exhibited a 44% increase in FFA titer. Co-overexpression of MMC and phosphoenolpyruvate carboxylase (PPC), which supplies the MMC precursor, further improved the titer by 40%. Expression of malic enzyme (MaeB) led to a 23% increase in FFA titer in the acetyl-CoA carboxylase (ACC)-overexpressing cells, but no increase in the MMC-overexpressing cells. The highest FFA production in the MMC-overexpressing strain was achieved through the addition of aspartic acid, which can be converted into oxaloacetate (OAA), resulting in a 120% increased titer compared with that in the ACC-overexpressing strain. These findings demonstrate that MMC provides an alternative pathway for malonyl-CoA synthesis and increases fatty acid production.
本研究考察了丙酸弗雷登斯氏菌的甲基丙二酰辅酶 A 羧基转移酶（MMC）对大肠杆菌中游离脂肪酸（FFA）产量的影响。MMC 的过表达使 FFA 产量增加了 44%。共表达 MMC 和磷酸烯醇丙酮酸羧化酶（PPC），为 MMC 的前体提供了补充，进一步将产量提高了 40%。苹果酸酶（MaeB）的表达使乙酰辅酶 A 羧化酶（ACC）过表达细胞中的 FFA 产量增加了 23%，但在 MMC 过表达细胞中没有增加。在 MMC 过表达菌株中，通过添加天冬氨酸可实现最高的 FFA 产量，天冬氨酸可转化为草酰乙酸（OAA），与 ACC 过表达菌株相比，产量增加了 120%。这些发现表明，MMC 提供了一种替代的丙二酰辅酶 A 合成途径，增加了脂肪酸的产量。
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