Institute for Management of Invasive Alien Species, 314 Yingdong teaching building, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, P. R. China.
Guangdong Institute of Applied Biological Resources, Guangzhou, 510260, P. R. China.
Sci Rep. 2017 Jun 8;7(1):3070. doi: 10.1038/s41598-017-03306-7.
Studies on insect olfaction have increased our understanding of insect's chemosensory system and chemical ecology, and have improved pest control strategies based on insect behavior. In this study, we assembled the antennal transcriptomes of the lychee giant stink bug, Tessaratoma papillosa, by using next generation sequencing to identify the major olfaction gene families in this species. In total, 59 odorant receptors, 14 ionotropic receptors (8 antennal IRs), and 33 odorant binding proteins (28 classic OBPs and 5 plus-C OBPs) were identified from the male and female antennal transcriptomes. Analyses of tissue expression profiles revealed that all 59 OR transcripts, 2 of the 8 antennal IRs, and 6 of the 33 OBPs were primarily expressed in the antennae, suggesting their putative role in olfaction. The sex-biased expression patterns of these antenna-predominant genes suggested that they may have important functions in the reproductive behavior of these insects. This is the first report that provides a comprehensive resource to future studies on olfaction in the lychee giant stink bug.
昆虫嗅觉研究增加了我们对昆虫化学生态学和化学感受系统的理解，并改进了基于昆虫行为的害虫防治策略。在这项研究中，我们通过使用下一代测序技术组装了荔枝蝽雄虫和雌虫的触角转录组，以鉴定该物种中的主要嗅觉基因家族。总共从雄虫和雌虫触角转录组中鉴定出了 59 个气味受体、14 个离子型受体（8 个触角 IRs）和 33 个气味结合蛋白（28 个经典 OBPs 和 5 个加-C OBPs）。组织表达谱分析表明，所有 59 个 OR 转录本、8 个触角 IRs 中的 2 个和 33 个 OBPs 中的 6 个主要在触角中表达，表明它们在嗅觉中可能具有潜在作用。这些主要在触角中表达的基因的性别偏表达模式表明，它们可能在这些昆虫的生殖行为中具有重要功能。这是首次报道为荔枝蝽的嗅觉研究提供了全面的资源。
