[Effects of FGF-21 on Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells in High Glucose Environment].

OBJECTIVES

To determine the effect of fibroblast growth factor-21(FGF-21)on the osteogenic differention of human bone mesenchymal stem cells (hBMSCs) exposed to a hyperglycemia condition .

METHODS

hBMSCs were isolated from adult bone marrows, and identified by Alizarin red and oil red O staining. The expressions of immunophenotype were analysed using flow cytometry (CD105, CD90, CD73, CD44).HBMSCs were divided into control group[glucose (Glu) concentration of 5.5 mmol/L], Glu A, B, C groups(Glu 16.5, 25, 40 mmol/L), FGF-21 group (Glu 5.5mmol/ L+ FGF-21 ),Glu B+ FGF-21 group, and Glu B +FGF-21+cell mitogen activated protein kinase (MAPK) blocker (PD98059, SP600125 ,and SB203580) groups. The effect of FGF-21 on the differentiation of hBMSCs was detected using indicators as follows: alkaline phosphatase(ALP)on day 14, mRNA expressions of, osteocalcin()and , protein expressions and phosphorylation of extracellular signal regulated kinase (ERK), mitogen-activated protein kinase(P38) and c-Jun N-terminal kinases(JNK) on day 21.

RESULTS

hBMSCs differentiated into osteoblast cells and lipocyte. The hBMSCs were identified by flow cytometry.Compared with control group, significant increases of mRNA, mRNA and mRNA levels, as well as phosphorylation of ERK, P38 and JNK were observed in Glu A, B, C groups.Compared with Glu B group, , and mRNA levels, and phosphorylation of ERK, P38 and JNK were decreased in Glu B+FGF-21 group .Compared with Glu B+FGF-21 group, and mRNA levels, and phosphorylation of ERK, JNK and P38 were decreased in Glu B +FGF-21 +MAPK blocker groups.

CONCLUSIONS

High glucose could promote the biomineralization of hBMSCs. FGF-21 in high glucose environment could inhibit the osteogenic differentiation of hBMSCs.