Li Xiao-Wan, Li Xiu-Guo, Takuwa Yoh, Cui Hong
Experimental Center of Fuctional Science, College of Medicine, Yanbian University, Yanji 133002,China.
Department of Physiology, Kanazawa University School of Medicine, Kanazawa 920-8640,Japan.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2016 Sep;47(5):714-717.
To determine the effect of sphingosine-1-phosphate receptor 2 (S1PR2) on vascular permeability in mice.
Acute lung injury models of mice were constructed with intra-tracheal administration of lipopolysaccharide (LPS) and compared with the controls with intra-tracheal administration of saline. The effect of S1PR2 on vascular permeability was observed by detecting leakage of Evans blue into lung tissues, pulmonary vascular leakage of fluorescein isothiocyanate (FITC)-dextran, and the wet/dry mass ratio of lungs. The effect of vascular endothelial growth factor (VEGF) on vascular endothelial permeability was detected by Miles analysis.
LPS injections induced significant Evans blue leakage, FITC-dextran pulmonary vascular leakage and pulmonary edema, which appeared to be more serious in S1PR2-deleted mice compared with those in wild-type mice. LPS enhanced Evans blue leakage associated with VEGF in a dose-dependent way in both S1PR2-deleted mice and wild type mice. But the vascular permeability response in subcutaneous tissues induced by VEGF was higher in S1PR2-deleted mice than that in wild-type mice.
S1PR2 is involved in endothelial cell barrier protections, which inhibits vascular permeability.
确定1-磷酸鞘氨醇受体2（S1PR2）对小鼠血管通透性的影响。
通过气管内给予脂多糖（LPS）构建小鼠急性肺损伤模型，并与气管内给予生理盐水的对照组进行比较。通过检测伊文思蓝渗入肺组织的情况、异硫氰酸荧光素（FITC）-葡聚糖的肺血管渗漏以及肺的湿/干质量比，观察S1PR2对血管通透性的影响。通过迈尔斯分析检测血管内皮生长因子（VEGF）对血管内皮通透性的影响。
注射LPS可导致显著的伊文思蓝渗漏、FITC-葡聚糖肺血管渗漏和肺水肿，与野生型小鼠相比，S1PR2基因敲除小鼠的这些表现似乎更严重。在S1PR2基因敲除小鼠和野生型小鼠中，LPS均以剂量依赖的方式增强与VEGF相关的伊文思蓝渗漏。但是，S1PR2基因敲除小鼠中由VEGF诱导的皮下组织血管通透性反应高于野生型小鼠。
S1PR2参与内皮细胞屏障保护，抑制血管通透性。
