Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629 HZ, Delft, The Netherlands.
Microbiology of Interfaces, Department River Ecology, Helmholtz Centre for Environmental Research - UFZ, Brueckstrasse 3A, 39114, Magdeburg, Germany.
Water Res. 2017 Oct 1;122:148-158. doi: 10.1016/j.watres.2017.05.068. Epub 2017 May 30.
A new acid soluble extracellular polymeric substance (acid soluble EPS) was extracted from an acetate fed aerobic granular sludge reactor operated at 35 °C. Acid soluble EPS dominated granules exhibited a remarkable and distinctive tangled tubular morphology. These granules are dominated by Defluviicoccus Cluster II organisms. Acetic acid instead of the usually required alkaline extraction medium was needed to dissolve the granules and solubilise the polymeric matrix. The extracted acid soluble EPS was analysed and identified using various instrumental analysis including H and C Nuclear Magnetic Resonance, Fourier Transform Infrared Spectroscopy and Raman spectroscopy. In addition, the glycoconjugates were characterized by fluorescence lectin-binding analysis. The acid soluble EPS is α-(1 → 4) linked polysaccharide, containing both glucose and galactose as monomers. There are OCH groups connected to the glucose monomer. Transmission and scanning electron microscopy (TEM, SEM) as well as confocal laser scanning microscopy (CLSM) showed that the acid soluble EPS was present as a tightly bound capsular EPS around bacterial cells ordered into a sarcinae-like growth pattern. The special granule morphology is decided by the acid soluble EPS produced by Defluviicoccus Cluster II organisms. This work shows that no single one method can be used to extract all possible extracellular polymeric substances. Results obtained here can support the elucidation of biofilm formation and structure in future research.
从在 35°C 下运行的乙酸进料好氧颗粒污泥反应器中提取了一种新的酸溶性胞外聚合物物质（酸溶性 EPS）。酸溶性 EPS 为主的颗粒表现出显著而独特的缠结管状形态。这些颗粒主要由 Defluviicoccus Cluster II 生物组成。需要乙酸而不是通常需要的碱性提取介质来溶解颗粒并溶解聚合物基质。使用包括 H 和 C 核磁共振、傅里叶变换红外光谱和拉曼光谱在内的各种仪器分析对提取的酸溶性 EPS 进行了分析和鉴定。此外，通过荧光凝集素结合分析对糖缀合物进行了表征。酸溶性 EPS 是α-（1→4）连接的多糖，其单体既包含葡萄糖又包含半乳糖。有 OCH 基团连接到葡萄糖单体上。透射电子显微镜（TEM）、扫描电子显微镜（SEM）和共聚焦激光扫描显微镜（CLSM）显示，酸溶性 EPS 作为一种紧密结合的荚膜 EPS 存在于细菌细胞周围，细菌细胞按 sarcinae 样生长模式排列。特殊的颗粒形态由 Defluviicoccus Cluster II 生物产生的酸溶性 EPS 决定。这项工作表明，没有一种单一的方法可以提取所有可能的胞外聚合物物质。这里获得的结果可以支持未来研究中生物膜形成和结构的阐明。
