Nam Hye Young, Min Kyung Hyun, Kim Da Eun, Choi Jeong Ryul, Lee Hong Jae, Lee Sang Cheon
Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea.
Department of Life and Nanopharmaceutical Science, Graduate School, Kyung Hee University, Seoul 130-701, Republic of Korea.
Colloids Surf B Biointerfaces. 2017 Sep 1;157:215-222. doi: 10.1016/j.colsurfb.2017.05.077. Epub 2017 Jun 3.
We developed a calcium phosphate (CaP)-assembled polymer nanocarrier for intracellular doxorubicin (DOX) delivery based on a mussel-inspired mineralization approach. A DOX-loaded core-shell polymer nanoparticle (DOX-NP) consisting of a poly(3,4-dihydroxy-l-phenylalanine) (PDOPA) core and a poly (ethylene glycol) (PEG) shell was utilized as a nanotemplate for CaP mineralization. The mean hydrodynamic diameter of the DOX-loaded CaP-mineralized polymer nanoparticles (DOX-CaP-NPs) was 154.3nm. Energy-dispersive X-ray spectroscopy confirmed that the DOX-CaP-NPs contained substantial amounts of Ca and P, elements found only in the CaP mineral. The loading efficiency and content of DOX, estimated by fluorescence spectroscopy, were 54.0% and 10.8wt%, respectively. The CaP deposited in the PDOPA core domain enabled the DOX-CaP-NPs to maintain a robust structure and effectively inhibit DOX release at extracellular pH, whereas at endosomal pH, the CaP core dissolved to trigger a facilitated DOX release. The DOX-CaP-NPs may serve as robust nanocarriers with a high delivery efficacy for cancer chemotherapy.
我们基于受贻贝启发的矿化方法，开发了一种用于细胞内递送阿霉素（DOX）的磷酸钙（CaP）组装聚合物纳米载体。一种由聚（3,4-二羟基-L-苯丙氨酸）（PDOPA）核和聚（乙二醇）（PEG）壳组成的载有DOX的核壳聚合物纳米颗粒（DOX-NP）被用作CaP矿化的纳米模板。载有DOX的CaP矿化聚合物纳米颗粒（DOX-CaP-NPs）的平均流体动力学直径为154.
