重组铜绿假单胞菌菌株DAB产生的鼠李糖脂生物表面活性剂的特性及原油去除效果

Characterization of rhamnolipid biosurfactants produced by recombinant Pseudomonas aeruginosa strain DAB with removal of crude oil.

作者信息

He Chunqiu, Dong Wen, Li Jing, Li Yanpeng, Huang Chao, Ma Yanling

机构信息

Shaanxi Provincial Key Laboratory of Biotechnology, Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, 229 Taibai North Rd, Xi'an, 710069, Shaanxi, China.

出版信息

Biotechnol Lett. 2017 Sep;39(9):1381-1388. doi: 10.1007/s10529-017-2370-x. Epub 2017 Jun 9.

DOI:10.1007/s10529-017-2370-x

PMID:28600649

Abstract

OBJECTIVES

To improve rhamnolipid production and its potential application in removal of crude oil, the recombinant Pseudomonas aeruginosa strain DAB was constructed to enhance yield of rhamnolipids.

RESULTS

Strain DAB had a higher yield of 17.3 g rhamnolipids l in the removal process with crude oil as the sole carbon source than 10 g rhamnolipids l of wild-type strain DN1, where 1% crude oil was degraded more than 95% after 14 days cultivation. These rhamnolipids reduced the surface tension of water from 72.92 to 26.15 mN m with CMC of 90 mg l. The predominant rhamnolipid congeners were Rha-C-C and Rha-Rha-C-C detected by MALDI-TOF MS analysis with approx. 70% relative abundance, although a total of 21 rhamnolipid congeners were accumulated.

CONCLUSION

Increasing the copy number of rhlAB genes efficiently enhanced the production of rhamnolipids by the recombinant P. aeruginosa DAB and thus presents a promising application for the bioremediation process.

摘要

目的

为提高鼠李糖脂产量及其在原油去除中的潜在应用，构建重组铜绿假单胞菌菌株DAB以提高鼠李糖脂产量。

结果

在以原油为唯一碳源的去除过程中，菌株DAB的鼠李糖脂产量较高，为17.3克/升，高于野生型菌株DN1的10克/升，在培养14天后，1%的原油降解率超过95%。这些鼠李糖脂将水的表面张力从72.92毫牛顿/米降低到26.15毫牛顿/米，临界胶束浓度为90毫克/升。通过基质辅助激光解吸电离飞行时间质谱分析检测到的主要鼠李糖脂同系物是鼠李糖-碳-碳和鼠李糖-鼠李糖-碳-碳，相对丰度约为70%，尽管总共积累了21种鼠李糖脂同系物。