Trangoni Marcos D, Gioffré Andrea K, Cravero Silvio L
Institute of Biotechnology, Center for Research in Veterinary and Agronomic Sciences - National Institute of Agricultural Technology (INTA), N. Repetto y de Los Reseros, 1686, Hurlingham, Buenos Aires, Argentina.
National Scientific and Technical Research Council (CONICET), Buenos Aires, Argentina.
Methods Mol Biol. 2017;1616:221-229. doi: 10.1007/978-1-4939-7037-7_14.
LAMP (loop-mediated isothermal amplification) is an isothermal nucleic acid amplification technique that is characterized by its efficiency, rapidity, high yield of final product, robustness, sensitivity, and specificity, with the blueprint that it can be implemented in laboratories of low technological complexity. Despite the conceptual complexity underlying the mechanistic basis for the nucleic acid amplification, the technique is simple to use and the amplification and detection can be carried out in just one step. In this chapter, we present a protocol based on LAMP for the rapid identification of isolates of Brucella spp. and Mycobacterium avium subsp. paratuberculosis, two major bacterial pathogens in veterinary medicine.
环介导等温扩增（LAMP）是一种等温核酸扩增技术，其特点是高效、快速、终产物产量高、稳健、灵敏且特异，其设想是可在技术复杂性较低的实验室中实施。尽管核酸扩增的机制基础存在概念上的复杂性，但该技术使用简单，扩增和检测可一步完成。在本章中，我们介绍一种基于LAMP的方案，用于快速鉴定布鲁氏菌属和副结核分枝杆菌的分离株，这是兽医学中的两种主要细菌病原体。
