Haematology Department, Calvary Mater Newcastle, NSW, Australia; School of Medicine and Public Health, University of Newcastle, NSW, Australia; Pathology North-Hunter, New Lambton Heights, NSW, Australia; Hunter Medical Research Institute, New Lambton, Australia; Hunter Cancer Research Alliance, Waratah, NSW, Australia.
Haematology Department, Calvary Mater Newcastle, NSW, Australia.
Thromb Res. 2017 Aug;156:65-72. doi: 10.1016/j.thromres.2017.04.019. Epub 2017 Apr 20.
Characterization of circulating microvesicles (MV) in healthy subjects in relation to various biological factors is not well studied.
We evaluated the influence of age, gender, smoking status, lipid and hormone profiles on circulating MV in healthy subjects.
Platelet free plasma from 143 volunteer blood donors (males=80, females=63) was evaluated by standardized flow cytometry for MV expressing CD41 (platelet-derived), CD105 (endothelial-derived), CD235 (red cell-derived), TF (tissue factor) and phosphatidylserine (PS) MV. Procoagulant function was measured by the Xa based assay (XaCT) and endogenous thrombin potential (ETP) using thrombin generation assay.
Those ≤29years and ≥60years had higher levels of MV subsets (CD41, CD235, TF and PS) compared to those aged 30-59years. The median CD41, CD105, CD235, TF and PS expressing MV by flow cytometry were similar or lower in females, whilst procoagulant activity by the XaCT assay was higher (p=0.002). In smokers (n=21), certain MV subsets (CD41, TF and PS) and functional activity (ETP) was lower (p<0.05). Regression analysis showed that MV parameters of CD41, CD105, TF and ETP could be predicted independently by age, whilst smoking predicted for CD105, CD235, TF, PS and ETP. Certain MV parameters also correlated with BMI, lipid and hormone levels. The small RNA and miRNA levels did not differ by age group, smoking status or gender.
It is important to recognize that differences may arise depending on age, gender, BMI, lipid, hormone levels and smoking status in apparently healthy subjects when evaluating MV for pathogenic potential.
目前对于健康个体中循环微泡（MV）与各种生物因素之间的关系还没有进行很好的研究。
我们评估了年龄、性别、吸烟状况、血脂和激素谱对健康个体循环 MV 的影响。
通过标准化流式细胞术，对来自 143 名志愿者献血者（男性 80 名，女性 63 名）的无血小板血浆进行 MV 表达 CD41（血小板衍生）、CD105（内皮衍生）、CD235（红细胞衍生）、TF（组织因子）和 PS（磷脂酰丝氨酸）MV 的评估。通过 Xa 基础测定法（XaCT）和血栓生成测定法测量的内源性凝血酶潜能（ETP）来评估促凝功能。
与 30-59 岁年龄组相比，≤29 岁和≥60 岁的 MV 亚群（CD41、CD235、TF 和 PS）水平更高。流式细胞术检测到的 CD41、CD105、CD235、TF 和 PS 表达的 MV 中位数在女性中相似或更低，而 XaCT 测定的促凝活性更高（p=0.002）。在吸烟者（n=21）中，某些 MV 亚群（CD41、TF 和 PS）和功能活性（ETP）降低（p<0.05）。回归分析显示，MV 参数 CD41、CD105、TF 和 ETP 可以独立地由年龄预测，而吸烟可以预测 CD105、CD235、TF、PS 和 ETP。某些 MV 参数也与 BMI、血脂和激素水平相关。小 RNA 和 miRNA 水平在年龄组、吸烟状态或性别之间没有差异。
在评估具有潜在致病性的 MV 时，重要的是要认识到，在年龄、性别、BMI、血脂、激素水平和吸烟状况方面，在看似健康的个体中可能会出现差异。
