Kucher A N, Nazarenko M S, Markov A V, Koroleva I A, Barbarash O L
Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, 634050, Russia.
Biochemistry (Mosc). 2017 Jun;82(6):698-706. doi: 10.1134/S0006297917060062.
In this study, we for the first time described the variability of methylation levels of 71 CpG sites in microRNA genes in leukocytes and blood vessels (coronary artery atherosclerotic plaques, intact internal thoracic arteries, and great saphenous veins) in patients with atherosclerosis using the Infinium HumanMethylation27 BeadChip microarray. Most of the analyzed CpG sites were characterized by the low variability, and most of these low-variable sites were hypomethylated in all tissue samples. CpG sites in coronary artery atherosclerotic plaques and leukocytes were similar in their methylation status. The highest variability of CpG methylation levels between different tissues was found for the CpG sites of the MIR10B gene; the methylation levels of these sites in leukocytes and atherosclerotic arteries were lower than in intact blood vessels. We also found that several cardiovascular disease risk factors, as well as medications, might affect methylation levels of CpG sites in microRNAs.
在本研究中，我们首次使用Infinium HumanMethylation27 BeadChip微阵列描述了动脉粥样硬化患者白细胞和血管（冠状动脉粥样硬化斑块、完整的胸廓内动脉和大隐静脉）中71个微小RNA基因中CpG位点甲基化水平的变异性。大多数分析的CpG位点变异性较低，并且这些低变异性位点在所有组织样本中大多处于低甲基化状态。冠状动脉粥样硬化斑块和白细胞中的CpG位点甲基化状态相似。在MIR10B基因的CpG位点中发现不同组织间CpG甲基化水平的变异性最高；这些位点在白细胞和动脉粥样硬化动脉中的甲基化水平低于完整血管中的甲基化水平。我们还发现，几种心血管疾病风险因素以及药物可能会影响微小RNA中CpG位点的甲基化水平。
