Martins Edenilce de Fátima Ferreira, Streit Danilo Pedro, Abreu Janessa Sampaio de, Corrêa-Filho Ruy Alberto Caetano, Oliveira Carlos Antonio Lopes de, Lopera-Barrero Nelson Mauricio, Povh Jayme Aparecido
Animal Science Graduate Program, Federal University of Mato Grosso, Cuiabá, MT, Brazil.
Department of Animal Science, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Theriogenology. 2017 Aug;98:57-61. doi: 10.1016/j.theriogenology.2017.04.047. Epub 2017 Apr 30.
The objective of this study was to evaluate Ovopel and carp pituitary extract (CPE) in the reproductive induction of Colossoma macropomum males. Nine treatments were tested in triplicate, totaling 27 experimental units. C. macropomum breeders were subjected to the following treatments: 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, and 0.7 Ovopel pellet/kg; 2.5 mg CPE/kg (traditional protocol); and a control treatment (no hormone). Breeders under hormone treatment produced a larger (P < 0.05) semen volume (2.4 ± 0.7 to 4.2 ± 0.3 mL) compared with the control (0.9 ± 0.4 mL). Sperm concentration did not differ significantly among treatments (7.2 × 10 ± 1.7 to 10.8 × 10 ± 2.6 spermatozoa/mL). Total sperm count was higher (P < 0.05) after treatment with 0.3, 0.4, and 0.6 Ovopel pellet/kg (41.6 ± 9.3 to 42.3 ± 10.5 × 10 spermatozoa) than the other Ovopel treatments (20.0 ± 2.4 to 26.9 ± 8.2 × 10 spermatozoa) and control (6.6 ± 1.1 × 10 spermatozoa), but did not differ significantly from CPE (33.7 ± 3.2 × 10 spermatozoa). Sperm motility was higher (P < 0.05) in the CPE treated, and 0.2, 0.3, and 0.7 Ovopel pellet/kg (88.3 ± 2.9 to 90.0 ± 5.0) breeders when compared with the other treatments (70.0 ± 10.0 to 78.3 ± 5.8), except for the 0.4 pellet/kg (81.7 ± 2.9) treatment, which did not differ significantly from any of the treatments. The motility period of the spermatozoa did not differ significantly among treatments (93.5 ± 15.7 to 120.0 ± 7.6 s). For the sperm morphological analysis, occurrence of normal spermatozoa was similar across the treatments, with three sperm abnormalities (short tail, bent tail, and detached head) differing (P < 0.05) among the treatments. Ovopel efficiently induced reproduction of C. macropomum breeders, with treatment using 0.3 and 0.4 Ovopel pellet/kg and CPE providing the best semen characteristics.
本研究的目的是评估奥沃佩尔（Ovopel）和鲤鱼脑垂体提取物（CPE）对巨脂鲤（Colossoma macropomum）雄鱼繁殖诱导的效果。试验设置了9种处理，每种处理重复3次，共27个实验单元。对巨脂鲤亲鱼进行以下处理：每千克体重分别注射0.1、0.2、0.3、0.4、0.5、0.6和0.7粒奥沃佩尔药丸；每千克体重注射2.5毫克CPE（传统方案）；以及一个对照处理（不使用激素）。与对照组（0.9±0.4毫升）相比，接受激素处理的亲鱼精液量更大（P<0.05）（2.4±0.7至4.2±0.3毫升）。各处理间精子浓度无显著差异（7.2×10±1.7至10.8×10±2.6个精子/毫升）。每千克体重注射0.3、0.4和0.6粒奥沃佩尔药丸处理后（41.6±9.3至42.3±10.5×10个精子），总精子数高于其他奥沃佩尔处理组（20.0±2.4至26.9±8.2×10个精子）和对照组（6.6±1.1×10个精子），但与CPE处理组（33.7±3.2×10个精子）无显著差异。与其他处理组（70.0±10.0至78.3±5.8）相比，CPE处理组以及每千克体重注射0.2、0.3和0.7粒奥沃佩尔药丸的亲鱼精子活力更高（P<0.05），除了每千克体重注射0.4粒药丸的处理组（81.7±2.9），其与其他任何处理组均无显著差异。各处理间精子活力持续时间无显著差异（93.5±15.7至120.0±7.6秒）。在精子形态分析中，各处理组正常精子的出现情况相似，但三种精子异常情况（短尾、弯尾和头部脱落）在各处理组间存在差异（P<0.05）。奥沃佩尔能有效诱导巨脂鲤亲鱼繁殖，每千克体重使用0.3和0.4粒奥沃佩尔药丸以及CPE处理能提供最佳精液特性。
