School of Medical and Allied Sciences, K.R. Mangalam University, Gurgaon, Haryana, India.
Umm Al-Qura University, Mecca, Saudi Arabia.
Int J Biol Macromol. 2017 Nov;104(Pt A):758-767. doi: 10.1016/j.ijbiomac.2017.05.129. Epub 2017 Jun 8.
Due to unique inherent catalytic characteristics of different size, shape and surface functionalized gold nanoparticles, their potential applications, are being explored in various fields such as drug delivery, biosensor, diagnosis and theranostics. However conventional process for synthesis of these metallic nanoparticles utilizes toxic reagents as reducing agents, additional capping agent for stability as well as surface functionalization for drug delivery purposes. Hence, in this work suitability of gum Ghatti for reducing, capping and surface functionalization during the synthesis of stable Gold nanoparticles were duly explored. Role and impact of key process variables i.e. volume of chloroauric acid solution, gum solution and temperature at their respective three different levels, as well as mechanism of formation of optimized gold nanoparticles were also investigated using Box- Behnken design. These novel synthesized optimized Gold nanoparticles were further characterized by UV spectrophotometer for its surface plasmon resonance (SPR) at around ∼530nm, dynamic light scattering (DLS) for its hydrodynamic size (112.5nm), PDI (0.222) and zeta potential (-21.3mV) while, transmission electron microscopy (TEM) further revealed surface geometry of these nanoparticles being spherical in shape.
由于不同尺寸、形状和表面功能化的金纳米粒子具有独特的固有催化特性，它们在药物输送、生物传感器、诊断和治疗等各个领域的潜在应用正在被探索。然而，这些金属纳米粒子的传统合成方法使用有毒试剂作为还原剂，使用额外的稳定剂作为稳定剂，以及使用表面功能化作为药物输送的目的。因此，在这项工作中，恰特草胶适用于在合成稳定的金纳米粒子时进行还原、封端和表面功能化。使用 Box-Behnken 设计研究了关键工艺变量（即氯金酸溶液、胶溶液的体积和温度在其三个不同水平下的作用和影响）以及优化金纳米粒子形成的机制。这些新型合成的优化金纳米粒子进一步通过紫外分光光度计在约 530nm 处的表面等离子体共振（SPR）、动态光散射（DLS）测量其水动力尺寸（112.5nm）、PDI（0.222）和zeta 电位（-21.3mV）进行了表征，而透射电子显微镜（TEM）进一步揭示了这些纳米粒子的表面几何形状为球形。
