Physiological Science Department, Federal University of Sao Carlos, Av. Washington Luiz, Km 235, 13565-905, Sao Carlos, Sao Paulo, Brazil.
Biological Science Department, Federal University of Espirito Santo, Av. Fernando Ferrari, 514, 29075-910, Vitoria, Espirito Santo, Brazil.
Chemosphere. 2017 Oct;184:309-317. doi: 10.1016/j.chemosphere.2017.06.007. Epub 2017 Jun 3.
Biological process treatment of landfill leachate produces a significant amount of sludge, characterized by high levels of organic matter from which humic acids are known to activate several enzymes of energy metabolism, stimulating plant growth. This study aimed to characterize humic acids extracted from landfill sludge and assess the effects on plants exposed to different concentrations (0.5, 1, 2 and 4 mM C L) by chemical and biological analysis, to elucidate the influence of such organic material and minimize potential risks of using sludge in natura. Landfill humic acids showed high carbon and nitrogen levels, which may represent an important source of nutrients for plants. Biochemical analysis demonstrated an increase of enzyme activity, especially H-ATPase in 2 mM C L landfill humic acid. Additionally, cytogenetic alterations were observed in meristematic and F cells, through nuclear abnormalities and micronuclei. Multivariate statistical analysis provided integration of physical, chemical and biological data. Despite all the nutritional benefits of humic acids and their activation of plant antioxidant systems, the observed biological effects showed concerning levels of mutagenicity.
生物处理过程会产生大量的垃圾渗滤液污泥，其特点是有机物含量高，已知腐殖酸可以激活几种能量代谢酶，从而刺激植物生长。本研究旨在对从垃圾渗滤液污泥中提取的腐殖酸进行表征，并通过化学和生物分析评估不同浓度（0.5、1、2 和 4 mM C L）对植物的影响，以阐明这种有机物质的影响，并最大限度地降低将污泥用于自然环境的潜在风险。垃圾渗滤液腐殖酸的碳氮含量较高，这可能代表植物的重要营养来源。生化分析表明，在 2 mM C L 垃圾渗滤液腐殖酸中，酶活性，特别是 H-ATPase 增加。此外，通过核异常和微核观察到分生细胞和 F 细胞的细胞遗传学改变。多元统计分析整合了物理、化学和生物数据。尽管腐殖酸具有所有的营养益处，并且可以激活植物的抗氧化系统，但观察到的生物效应显示出令人担忧的诱变水平。
