Khan Muhammad Tanveer, Dalvin Sussie, Nilsen Frank, Male Rune
Departments of Biology University of Bergen, N-5020 Bergen, Norway.
Sea Lice Research Centre, Institute of Marine Research, 5817 Bergen, Norway.
J Lipid Res. 2017 Aug;58(8):1613-1623. doi: 10.1194/jlr.M076430. Epub 2017 Jun 10.
The salmon louse, , is an endemic ectoparasite on salmonid fish that is challenging for the salmon farming industry and wild fish. Salmon lice produce high numbers of offspring, necessitating sequestration of large amounts of lipids into growing oocytes as a major energy source for larvae, most probably mediated by lipoproteins. The microsomal triglyceride transfer protein (MTP) is essential for the assembly of lipoproteins. Salmon lice have three MTP () transcript variants encoding two different protein isoforms, which are predicted to contain three β-sheets (N, C, and A) and a central helical domain, similar to MTPs from other species. In adult females, the LsMTPs are differently transcribed in the sub-cuticular tissues, the intestine, the ovary, and in the mature eggs. RNA interference-mediated knockdown of in mature females gave offspring with significantly fewer neutral lipids in their yolk and only 10-30% survival. The present study suggests the importance of LsMTP in reproduction and lipid metabolism in adult female , a possible metabolic bottleneck that could be exploited for the development of new anti-parasitic treatment methods.
鲑鱼虱是鲑科鱼类特有的体表寄生虫，对鲑鱼养殖业和野生鱼类构成挑战。鲑鱼虱繁殖力强，需要将大量脂质隔离到正在生长的卵母细胞中，作为幼虫的主要能量来源，这很可能是由脂蛋白介导的。微粒体甘油三酯转移蛋白（MTP）对脂蛋白的组装至关重要。鲑鱼虱有三种MTP（）转录变体，编码两种不同的蛋白质异构体，预计它们含有三个β折叠（N、C和A）和一个中央螺旋结构域，类似于其他物种的MTP。在成年雌性鲑鱼虱中，LsMTPs在表皮下组织、肠道、卵巢和成熟卵中的转录情况不同。RNA干扰介导的成年雌性鲑鱼虱中基因敲除，导致其后代卵黄中的中性脂质显著减少，存活率仅为10%-30%。本研究表明LsMTP在成年雌性鲑鱼虱的繁殖和脂质代谢中具有重要作用，这可能是一个潜在的代谢瓶颈，可用于开发新的抗寄生虫治疗方法。
