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犬Wilms瘤1的分子克隆用于犬组织的免疫组织化学分析。

Molecular cloning of canine Wilms' tumor 1 for immunohistochemical analysis in canine tissues.

作者信息

Sakai Osamu, Sakurai Masashi, Sakai Hiroki, Kubo Masahito, Hiraoka Hiroko, Baba Kenji, Okuda Masaru, Mizuno Takuya

机构信息

Laboratory of Molecular Diagnostics and Therapeutics, The United Graduate School of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan.

Laboratory of Veterinary Pathology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan.

出版信息

J Vet Med Sci. 2017 Jul 28;79(7):1272-1277. doi: 10.1292/jvms.17-0229. Epub 2017 Jul 12.

DOI:10.1292/jvms.17-0229
PMID:28603218
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5559376/
Abstract

Wilms' tumor 1 (WT1) expression has been investigated in various human cancers as a target molecule for cancer immunotherapy. However, few studies have focused on WT1 expression in dogs. Firstly, cDNA of canine WT1 (cWT1) was molecularly cloned from normal canine kidney. The cross-reactivity of the anti-human WT1 monoclonal antibody (6F-H2) with cWT1 was confirmed via Western blotting using cells overexpressing cWT1. Immunohistochemical staining revealed that cWT1 expression was detected in all canine lymphoma tissues and in some normal canine tissues, including the kidney and lymph node. cWT1 is a potential immunotherapy target against canine cancers.

摘要

威尔姆斯瘤1（WT1）表达已在多种人类癌症中作为癌症免疫治疗的靶分子进行了研究。然而，很少有研究关注WT1在犬类中的表达。首先，从正常犬肾中分子克隆出犬WT1（cWT1）的cDNA。通过使用过表达cWT1的细胞进行蛋白质免疫印迹，证实了抗人WT1单克隆抗体（6F-H2）与cWT1的交叉反应性。免疫组织化学染色显示，在所有犬淋巴瘤组织以及包括肾脏和淋巴结在内的一些正常犬组织中均检测到cWT1表达。cWT1是针对犬类癌症的潜在免疫治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85f4/5559376/6a5e98b7f891/jvms-79-1272-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85f4/5559376/faac065e08fe/jvms-79-1272-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85f4/5559376/ee98f05e3e57/jvms-79-1272-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85f4/5559376/810593fbaf0f/jvms-79-1272-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85f4/5559376/6a5e98b7f891/jvms-79-1272-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85f4/5559376/faac065e08fe/jvms-79-1272-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85f4/5559376/ee98f05e3e57/jvms-79-1272-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85f4/5559376/810593fbaf0f/jvms-79-1272-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85f4/5559376/6a5e98b7f891/jvms-79-1272-g004.jpg

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WT1 vaccination in AML and MDS: A pilot trial with synthetic analog peptides.WT1疫苗用于急性髓系白血病和骨髓增生异常综合征：一项合成类似物肽的试点试验。
Am J Hematol. 2015 Jul;90(7):602-7. doi: 10.1002/ajh.24014. Epub 2015 May 3.
2
WT1 and Pax2 re-expression is required for epithelial-mesenchymal transition in 5/6 nephrectomized rats and cultured kidney tubular epithelial cells.WT1 和 Pax2 的重新表达是 5/6 肾切除大鼠和培养的肾小管上皮细胞上皮-间充质转化所必需的。
Cells Tissues Organs. 2012;195(4):296-312. doi: 10.1159/000327530. Epub 2011 Jul 19.
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Calreticulin expression in neoplastic versus normal dog mammary glands: a cDNA subtraction-based study.
肿瘤与正常犬乳腺组织中钙网织蛋白的表达：基于 cDNA 消减的研究。
Res Vet Sci. 2012 Feb;92(1):80-91. doi: 10.1016/j.rvsc.2010.10.028. Epub 2010 Dec 3.
4
WT1 peptide vaccine induces reduction in minimal residual disease in an Imatinib-treated CML patient.WT1 肽疫苗可诱导伊马替尼治疗的 CML 患者微小残留病灶减少。
Eur J Haematol. 2010 Oct;85(4):358-60. doi: 10.1111/j.1600-0609.2010.01497.x. Epub 2010 Aug 30.
5
WT1 peptide vaccinations induce CD4 and CD8 T cell immune responses in patients with mesothelioma and non-small cell lung cancer.WT1 肽疫苗可诱导间皮瘤和非小细胞肺癌患者的 CD4 和 CD8 T 细胞免疫应答。
Cancer Immunol Immunother. 2010 Oct;59(10):1467-79. doi: 10.1007/s00262-010-0871-8. Epub 2010 Jun 8.
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A vaccine targeting telomerase enhances survival of dogs affected by B-cell lymphoma.一种针对端粒酶的疫苗可提高 B 细胞淋巴瘤犬的存活率。
Mol Ther. 2010 Aug;18(8):1559-67. doi: 10.1038/mt.2010.104. Epub 2010 Jun 8.
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WT1 peptide vaccination in combination with imatinib therapy for a patient with CML in the chronic phase.WT1 肽疫苗接种联合伊马替尼治疗慢性期 CML 患者。
Int J Med Sci. 2010 Apr 20;7(2):72-81. doi: 10.7150/ijms.7.72.
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