犬Wilms瘤1的分子克隆用于犬组织的免疫组织化学分析。

Molecular cloning of canine Wilms' tumor 1 for immunohistochemical analysis in canine tissues.

作者信息

Sakai Osamu, Sakurai Masashi, Sakai Hiroki, Kubo Masahito, Hiraoka Hiroko, Baba Kenji, Okuda Masaru, Mizuno Takuya

机构信息

Laboratory of Molecular Diagnostics and Therapeutics, The United Graduate School of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan.

Laboratory of Veterinary Pathology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan.

出版信息

J Vet Med Sci. 2017 Jul 28;79(7):1272-1277. doi: 10.1292/jvms.17-0229. Epub 2017 Jul 12.

DOI:10.1292/jvms.17-0229

PMID:28603218

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5559376/

Abstract

Wilms' tumor 1 (WT1) expression has been investigated in various human cancers as a target molecule for cancer immunotherapy. However, few studies have focused on WT1 expression in dogs. Firstly, cDNA of canine WT1 (cWT1) was molecularly cloned from normal canine kidney. The cross-reactivity of the anti-human WT1 monoclonal antibody (6F-H2) with cWT1 was confirmed via Western blotting using cells overexpressing cWT1. Immunohistochemical staining revealed that cWT1 expression was detected in all canine lymphoma tissues and in some normal canine tissues, including the kidney and lymph node. cWT1 is a potential immunotherapy target against canine cancers.

摘要

威尔姆斯瘤1（WT1）表达已在多种人类癌症中作为癌症免疫治疗的靶分子进行了研究。然而，很少有研究关注WT1在犬类中的表达。首先，从正常犬肾中分子克隆出犬WT1（cWT1）的cDNA。通过使用过表达cWT1的细胞进行蛋白质免疫印迹，证实了抗人WT1单克隆抗体（6F-H2）与cWT1的交叉反应性。免疫组织化学染色显示，在所有犬淋巴瘤组织以及包括肾脏和淋巴结在内的一些正常犬组织中均检测到cWT1表达。cWT1是针对犬类癌症的潜在免疫治疗靶点。