Department of Chemical and Biological Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.
KU-KIST Graduate School of Converging Science and Technology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.
Chem Asian J. 2017 Aug 17;12(16):2033-2037. doi: 10.1002/asia.201700769. Epub 2017 Jul 4.
The immunochromatographic assay (ICA) using a nitrocellulose (NC) membrane offers several advantages. This technique is a rapid and straightforward method in contrast to other immunoassays. Polydiacetylene (PDA) vesicles have unique optical properties, displaying red color and red fluorescence at the same time. In this system, red-phase PDA vesicles are used as a fluorescent dye as well as a surface for immobilized hepatitis B surface antibody (HBsAb). PDA has a remarkable stability compared with other fluorescent dyes. In this study, the most suitable PDA/HBsAb complexes are introduced for detecting hepatitis B surface antigen (HBsAg). Then, the PDA/HBsAb complexes affixed antibody is attached to NC membrane, which has two lines to confirm detection of HBsAg. The main advantage of this system is that the detection of HBsAg can be observed in both visible and fluorescent images due to the optical properties of polydiacetylene. Detection of HBsAg is observed up to 0.1 ng mL by fluorescent analysis and confirmed by red line on the NC membrane up to 1 ng mL (HBsAg) using the naked eye. Consequently, these results show that PDA/HBsAb complexes were successfully applied to ICA for the diagnosis of hepatitis B.
免疫层析分析（ICA）使用硝酸纤维素（NC）膜具有许多优点。与其他免疫分析相比，该技术是一种快速而直接的方法。聚二乙炔（PDA）囊泡具有独特的光学特性，同时显示红色和红色荧光。在该系统中，红色相 PDA 囊泡既可用作荧光染料，也可用作固定乙肝表面抗体（HBsAb）的表面。与其他荧光染料相比，PDA 具有显著的稳定性。在这项研究中，引入了最适合检测乙肝表面抗原（HBsAg）的 PDA/ HBsAb 复合物。然后，将固定有抗体的 PDA/ HBsAb 复合物附着到 NC 膜上，NC 膜上有两条线用于确认 HBsAg 的检测。该系统的主要优点是由于聚二乙炔的光学特性，可以在可见和荧光图像中观察到 HBsAg 的检测。荧光分析可检测到 0.1ng/mL 的 HBsAg，并且通过肉眼可以在 NC 膜上检测到高达 1ng/mL（HBsAg）的红线来确认检测。因此，这些结果表明 PDA/ HBsAb 复合物已成功应用于 ICA 诊断乙型肝炎。
