Gilpin D F, Nixon K A, Bull M, McGrath S J, Sherrard L, Rolain J M, Mahenthiralingam E, Elborn J S, Tunney M M
School of Pharmacy, Queen's University of Belfast, Belfast, Northern Ireland, UK.
Cardiff School of Biosciences, Cardiff University, Wales, UK.
J Med Microbiol. 2017 Jun;66(6):825-832. doi: 10.1099/jmm.0.000500.
spp. represent a diverse genus of bacteria, frequently identified by both culture and molecular methods in the lungs of patients with chronic respiratory infection. However, their role in the pathogenesis of chronic lung infection is unclear; therefore, a more complete understanding of their molecular epidemiology is required. Pulsed Field Gel Electrophoresis (PFGE) and Random Amplified Polymorphic DNA (RAPD) assays were developed and used to determine the degree of similarity between sequential isolates (=42) from cystic fibrosis (CF) patients during periods of clinical stability and exacerbation. A wide diversity of PFGE and RAPD banding patterns were observed, demonstrating considerable within-genus heterogeneity. In 8/12 (66.7 %) cases, where the same species was identified at sequential time points, pre- and post-antibiotic treatment of an exacerbation, PFGE/RAPD profiles were highly similar or identical. Congruence was observed between PFGE and RAPD (adjusted Rand coefficient, 0.200; adjusted Wallace 0.459, 0.128). Furthermore, some isolates could not be adequately assigned a species name on the basis of 16S rRNA analysis: these isolates had identical PFGE/RAPD profiles to . The similarity in PFGE and RAPD banding patterns observed in sequential CF isolates may be indicative of the persistence of this genus in the CF lung. Further work is required to determine the clinical significance of this finding, and to more accurately distinguish differences in pathogenicity between species.
某些菌属代表了一类多样的细菌，在慢性呼吸道感染患者的肺部，常通过培养和分子方法进行鉴定。然而，它们在慢性肺部感染发病机制中的作用尚不清楚；因此，需要对其分子流行病学有更全面的了解。脉冲场凝胶电泳（PFGE）和随机扩增多态性DNA（RAPD）分析方法被开发出来，并用于确定来自囊性纤维化（CF）患者在临床稳定期和病情加重期的连续分离株（=42）之间的相似程度。观察到PFGE和RAPD条带模式具有广泛的多样性，表明该菌属内存在相当大的异质性。在12例中有8例（66.7%），在病情加重期抗生素治疗前后的连续时间点鉴定出相同的菌种，PFGE/RAPD图谱高度相似或相同。PFGE和RAPD之间观察到一致性（调整后的兰德系数，0.200；调整后的华莱士系数，0.459，0.128）。此外，一些分离株基于16S rRNA分析无法被充分指定菌种名称：这些分离株的PFGE/RAPD图谱与[具体菌种]相同。在连续的CF分离株中观察到的PFGE和RAPD条带模式的相似性可能表明该菌属在CF肺部持续存在。需要进一步开展工作来确定这一发现的临床意义，并更准确地区分不同菌种之间致病性的差异。
