Terry Sandra N, Manganaro Lara, Cuesta-Dominguez Alvaro, Brinzevich Daria, Simon Viviana, Mulder Lubbertus C F
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; The Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Virology. 2017 Sep;509:52-59. doi: 10.1016/j.virol.2017.06.004. Epub 2017 Jun 9.
The human endogenous retroviruses (HERV)-K of the HML-2 group include full-length or near full-length elements encoding functional proteins, and are classified as type-1 or type-2 (type-1 has a deletion in the 5' end of the env gene). Because proteins of different retroviruses can interact, we hypothesized that HERV-K envelope (Env) could influence HIV-1 replication. Here we describe the negative effect of envelope expression of certain type-2 HERV-Ks on HIV-1 production. All HIV-1 and SIV strains tested were susceptible to various degrees to inhibition by the HERV-K108 envelope. We identified four residues within HERV-K108 Env as being critical to inhibit HIV-1 production. No inhibition was observed on EGFP expression, indicating that HERV-K Env does not affect general protein production. These findings demonstrate that envelope proteins from some endogenous retroviruses can limit production of exogenous lentiviruses such as HIV-1. Future studies will elucidate the mechanism mediating HIV-1 inhibition by HERV Envs.
HML-2组的人类内源性逆转录病毒（HERV）-K包含编码功能蛋白的全长或接近全长元件，分为1型或2型（1型在env基因的5'端有缺失）。由于不同逆转录病毒的蛋白可以相互作用，我们推测HERV-K包膜（Env）可能影响HIV-1复制。在此，我们描述了某些2型HERV-K的包膜表达对HIV-1产生的负面影响。所有测试的HIV-1和SIV毒株都不同程度地易受HERV-K108包膜的抑制。我们确定HERV-K108 Env中的四个残基对抑制HIV-1产生至关重要。未观察到对EGFP表达的抑制，表明HERV-K Env不影响一般蛋白质的产生。这些发现表明，一些内源性逆转录病毒的包膜蛋白可以限制外源性慢病毒如HIV-1的产生。未来的研究将阐明HERV Env介导HIV-1抑制的机制。
