Pilaski J, Mackenstein H
Zentralbl Bakteriol Mikrobiol Hyg B. 1985 Mar;180(4):394-420.
Collecting during two periods, from September 4, 1979, to September 12, 1980, and from August 17 to September 10, 1981, a total of 45,705 mosquitoes was caught for virus isolation studies in 6 different regions in Germany and the Netherlands (Lower Rhine area, Upper Rhine area near Germersheim, Upper Main area near Baunach, Amper Moos), in Austria (eastern shore of Lake Neusiedl), and in Italy (Isonzo river delta). 25 mosquito species were identified belonging to the genera Aedes, Culex, Culiseta, Mansonia, and Uranotaenia. The relative mosquito species composition was determined for the different collecting sites. By intracerebral inoculation of 2 to 4 days old suckling mice with extracts of each mosquito pool 6 virus strains were isolated which were identified as Tahyna (TAH) virus strains using the indirect immunofluorescence technique in cell culture and the baby mouse neutralization test. The origin of these strains were the following regions: one TAH virus strain was isolated from Aedes caspius mosquitoes (1 of 158 pools) collected from 17. 8 to 30. 8. 81 at the eastern shore of Lake Neusiedl. 5 additional isolates were obtained from 6,066 mosquitoes (62 pools) collected on 9. and 10. 9. 81 in the Upper Rhine area near Germersheim (Isle Grün). Two of these virus strains were isolated from Aedes vexans and three from unidentified mosquitoes. This is the first TAH virus isolation from mosquitoes in the Upper Rhine area, which had been regarded by Ackermann and coworkers (1970) and Spieckermann and Ackermann (1974) as a potential natural TAH virus focus on the basis of serological studies in humans and sentinel rabbits during 1969. The isolation of TAH virus in the Lake Neusiedl area essentially confirms the results obtained by Aspöck and Kunz (1967) for this region. The possible influence of some ecological factors on the geographical distribution of TAH virus is discussed.
在两个时间段进行了采集，即从1979年9月4日至1980年9月12日，以及从1981年8月17日至9月10日，在德国和荷兰的6个不同地区（下莱茵地区、格默斯海姆附近的上莱茵地区、鲍纳赫附近的上美因地区、安珀沼泽）、奥地利（新锡德尔湖东岸）和意大利（伊松佐河三角洲）共捕获了45,705只蚊子用于病毒分离研究。鉴定出25种蚊子，分属于伊蚊属、库蚊属、脉毛蚊属、曼蚊属和蓝带蚊属。确定了不同采集地点的相对蚊子种类组成。通过用每批蚊子提取物对2至4日龄乳鼠进行脑内接种，分离出6株病毒，利用细胞培养中的间接免疫荧光技术和乳鼠中和试验将其鉴定为塔希纳（TAH）病毒株。这些毒株的来源如下地区：1株TAH病毒株从1981年8月17日至8月30日在新锡德尔湖东岸采集的158批里海伊蚊（1批）中分离得到。另外5株分离株从1981年9月9日和10日在格默斯海姆附近的上莱茵地区（格林岛）采集的6066只蚊子（62批）中获得。其中2株病毒株从骚扰伊蚊中分离得到，3株从未鉴定的蚊子中分离得到。这是首次在上莱茵地区的蚊子中分离出TAH病毒，基于1969年对人类和哨兵兔的血清学研究，阿克曼及其同事（1970年）以及施皮克曼和阿克曼（1974年）曾将该地区视为潜在的天然TAH病毒疫源地。在新锡德尔湖地区分离出TAH病毒基本上证实了阿斯波克和昆茨（19世纪67年代）在该地区获得的结果。讨论了一些生态因素对TAH病毒地理分布的可能影响。 （注：原文中1967表述有误，推测为1967年，译文按此修正）
