使用含低聚乙二醇的可光裂解赖氨酸标签高效合成及纯化β淀粉样蛋白（1-42）
The efficient synthesis and purification of amyloid-β(1-42) using an oligoethylene glycol-containing photocleavable lysine tag.
作者信息
Karas John A, Noor Asif, Schieber Christine, Connell Timothy U, Separovic Frances, Donnelly Paul S
机构信息
School of Chemistry and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, VIC 3010, Australia.
出版信息
Chem Commun (Camb). 2017 Jun 22;53(51):6903-6905. doi: 10.1039/c7cc03147e.
A short, monodisperse oligoethylene glycol-containing photocleavable lysine tag was developed to facilitate the efficient purification of hydrophobic and fibril-forming peptides. This new tag was used to prepare a modified Aβ peptide with increased solubility and decreased propensity to aggregate in aqueous media. The solubilising tag was readily removed by irradiation with UV light and permitted the preparation and isolation of Aβ in high purity and yield.
开发了一种短的、单分散的含低聚乙二醇的光可裂解赖氨酸标签，以促进疏水性和形成纤维的肽的高效纯化。这种新标签用于制备一种修饰的Aβ肽，其在水性介质中的溶解度增加且聚集倾向降低。通过紫外线照射可轻松去除增溶标签，并允许以高纯度和高产率制备和分离Aβ。
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