Department of Radiology, Tianjin Medical University General Hospital, Tianjin, China.
Medical Research Council Mitochondrial Biology Unit, Cambridge BioMedical Campus, Cambridge, UK.
Magn Reson Med. 2018 Mar;79(3):1559-1567. doi: 10.1002/mrm.26772. Epub 2017 Jun 12.
To investigate the effect of mitochondria-targeted antioxidant MitoQ in reducing the severity of renal ischemia-reperfusion injury (IRI) in rats using T -weighted imaging and dynamic contrast-enhanced MRI (DCE-MRI).
Ischemia-reperfusion injury was induced by temporarily clamping the left renal artery. Rats were pretreated with MitoQ or saline. The MRI examination was performed before and after IRI (days 2, 5, 7, and 14). The T -weighted standardized signal intensity of the outer stripe of the outer medulla (OSOM) was measured. The unilateral renal clearance rate k was derived from DCE-MRI. Histopathology was evaluated after the final MRI examination.
The standardized signal intensity of the OSOM on IRI kidneys with MitoQ were lower than those with saline on days 5 and 7 (P = 0.004, P < 0.001, respectively). K values of IRI kidneys with MitoQ were higher than those with saline at all time points (P = 0.002, P < 0.001, P = 0.001, P < 0.001). Histopathology showed that renal damage was the most predominant on the OSOM of IRI kidneys with saline, which was less obvious with MitoQ (P < 0.001).
These findings demonstrate that MitoQ can reduce the severity of renal damage in rodent IRI models using T -weighted imaging and DCE-MRI. Magn Reson Med 79:1559-1667, 2018. © 2017 The Authors Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
使用 T1 加权成像和动态对比增强磁共振成像（DCE-MRI）研究线粒体靶向抗氧化剂 MitoQ 对减轻大鼠肾缺血再灌注损伤（IRI）严重程度的作用。
通过暂时夹闭左肾动脉诱导 IRI。用 MitoQ 或盐水预处理大鼠。在 IRI 前后（第 2、5、7 和 14 天）进行 MRI 检查。测量外髓外带（OSOM）的 T1 加权标准化信号强度。从 DCE-MRI 得出单侧肾清除率 k。在最后一次 MRI 检查后评估组织病理学。
IRI 肾脏 MitoQ 组的 OSOM 标准化信号强度在第 5 和 7 天低于盐水组（P=0.004，P<0.001）。IRI 肾脏 MitoQ 组的 k 值在所有时间点均高于盐水组（P=0.002，P<0.001，P=0.001，P<0.001）。组织病理学显示，盐水组 IRI 肾脏 OSOM 肾损伤最严重，MitoQ 组则不那么明显（P<0.001）。
这些发现表明，使用 T1 加权成像和 DCE-MRI，MitoQ 可以减轻大鼠 IRI 模型中肾脏损伤的严重程度。磁共振医学 79:1559-1667，2018。© 2017 作者磁共振医学由 Wiley 期刊出版公司代表国际磁共振学会出版。这是在知识共享署名许可条款下的许可，允许在任何媒介中使用、分发和复制原作，只要原作者被正确引用。
