Grinkevich V A, Trubetskaia O E, Chertova E N, Murav'eva T I, Aldanova N A
Bioorg Khim. 1985 Mar;11(3):321-33.
Trypsin and cyanogen bromide were used for cleavage of the OSCP preparations. The peptide mixtures thus formed were separated into individual components by a combination of various chromatographic procedures: gel filtration, ion exchange and paper chromatography, as well as reversed-phase HPLC. As a result, 31 tryptic peptides and 9 out of 10 possible cyanogen bromide peptides were isolated. Determination of the amino acid sequences of these peptide allowed the alignment of cyanogen bromide fragments in the polypeptide chain that shed light on the "architecture" of the protein molecule as a whole. It also afforded the overlappings for tryptic peptides, 16 in the N-terminal and 8 in the C-terminal portions of the molecule.
用胰蛋白酶和溴化氰对寡霉素敏感相关蛋白（OSCP）制剂进行切割。由此形成的肽混合物通过多种色谱方法的组合分离成各个组分：凝胶过滤、离子交换、纸色谱以及反相高效液相色谱。结果，分离出了31个胰蛋白酶肽段和10个可能的溴化氰肽段中的9个。对这些肽段氨基酸序列的测定使得能够在多肽链中排列溴化氰片段，从而揭示整个蛋白质分子的“结构”。这也为胰蛋白酶肽段提供了重叠区域，在分子的N端有16个，C端有8个。
