Minchenko Dmytro O, Tsymbal D O, Yavorovsky O P, Solokha N V, Minchenko O H
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Endocr Regul. 2017 Apr 25;51(2):84-95. doi: 10.1515/enr-2017-0008.
The aim of the present study was to examine the effect of chromium disilicide and titanium nitride nanoparticles on the expression level of genes encoding important regulatory factors (IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP5, SNARK/NUAK2, CD36, and PECAM1/CD31) in mouse liver for evaluation of possible toxic effects of these nanoparticles.
Male mice received 20 mg chromium disilicide nanoparticles (45 nm) and titanium nitride nanoparticles (20 nm) with food every working day for 2 months. The expression of IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP5, SNARK, CD36, and PECAM1 genes in mouse liver was studied by quantitative polymerase chain reaction.
Treatment of mice with chromium disilicide nanoparticles led to down-regulation of the expression of IGFBP2, IGFBP5, PECAM1, and SNARK genes in the liver in comparison with control mice, with more prominent changes for SNARK gene. At the same time, the expression of IGFBP3 and CD36 genes was increased in mouse liver upon treatment with chromium disilicide nanoparticles. We have also shown that treatment with titanium nitride nanoparticles resulted in down-regulation of the expression of IGFBP2 and SNARK genes in the liver with more prominent changes for SNARK gene. At the same time, the expression of IGFBP3, IGFBP4, and CD36 genes was increased in the liver of mice treated with titanium nitride nanoparticles. Furthermore, the effect of chromium disilicide nanoparticles on IGFBP2 and CD36 genes expression was significantly stronger as compared to titanium nitride nanoparticles.
The results of this study demonstrate that chromium disilicide and titanium nitride nanoparticles have variable effects on the expression of IGFBP2, IGFBP3, IGFBP4, IGFBP5, SNARK, CD36, and PECAM1 genes in mouse liver, which may reflect the genotoxic activities of the studied nanoparticles.
本研究旨在检测二硅化铬和氮化钛纳米颗粒对小鼠肝脏中编码重要调节因子（胰岛素样生长因子结合蛋白1、胰岛素样生长因子结合蛋白2、胰岛素样生长因子结合蛋白3、胰岛素样生长因子结合蛋白4、胰岛素样生长因子结合蛋白5、SNARK/NUAK2、CD36和PECAM1/CD31）的基因表达水平的影响，以评估这些纳米颗粒可能的毒性作用。
雄性小鼠在每个工作日随食物摄入20毫克二硅化铬纳米颗粒（45纳米）和氮化钛纳米颗粒（20纳米），持续2个月。通过定量聚合酶链反应研究小鼠肝脏中胰岛素样生长因子结合蛋白1、胰岛素样生长因子结合蛋白2、胰岛素样生长因子结合蛋白3、胰岛素样生长因子结合蛋白4、胰岛素样生长因子结合蛋白5、SNARK、CD36和PECAM1基因的表达。
与对照小鼠相比，用二硅化铬纳米颗粒处理小鼠导致肝脏中胰岛素样生长因子结合蛋白2、胰岛素样生长因子结合蛋白5、PECAM1和SNARK基因的表达下调，其中SNARK基因的变化更为显著。同时，用二硅化铬纳米颗粒处理后，小鼠肝脏中胰岛素样生长因子结合蛋白3和CD36基因的表达增加。我们还表明，用氮化钛纳米颗粒处理导致肝脏中胰岛素样生长因子结合蛋白2和SNARK基因的表达下调，其中SNARK基因的变化更为显著。同时，用氮化钛纳米颗粒处理的小鼠肝脏中胰岛素样生长因子结合蛋白3、胰岛素样生长因子结合蛋白4和CD36基因的表达增加。此外，与氮化钛纳米颗粒相比，二硅化铬纳米颗粒对胰岛素样生长因子结合蛋白2和CD36基因表达的影响明显更强。
本研究结果表明，二硅化铬和氮化钛纳米颗粒对小鼠肝脏中胰岛素样生长因子结合蛋白2、胰岛素样生长因子结合蛋白3、胰岛素样生长因子结合蛋白4、胰岛素样生长因子结合蛋白5、SNARK、CD36和PECAM1基因的表达有不同影响，这可能反映了所研究纳米颗粒的遗传毒性活性。
