Mohebbati R, Hosseini M, Haghshenas M, Nazariborun A, Beheshti Farimah
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Endocr Regul. 2017 Apr 25;51(2):105-113. doi: 10.1515/enr-2017-0010.
We investigated the effects of hydroalcoholic extract of Nigella sativa (NS) on renal tissue oxidative damage associated with propylthiouracil (PTU)-induced hypothyroidism during neonatal and juvenile growth in rats.
Pregnant rats were divided into five groups designated as: 1) control; 2) propylthiouracil (PTU); 3) PTU-NS100; 4) PTU-NS200, and 5) PTU-NS400. All mothers except the control group received 0.005% PTU in their drinking water during lactation. Besides PTU, mothers in groups 3-5 received 100, 200, and 400 mg/kg of NS extract. After lactation period, the off spring continued to receive the same experimental treatment for the first 8 weeks of their life. Ten male off springs of each group were randomly selected, blood samples collected, and the kidney tissues removed.
The serum thyroxin concentration in PTU group was lower than control group and improved by extract. PTU increased the renal malondialdehyde (MDA), while reduced the total thiols concentrations and catalase (CAT) and superoxide dismutase (SOD) activity compared to control group. Administration of 200 and 400 mg/kg of NS extract decreased MDA level, while it increased the total thiols and 400 mg/kg increased CAT and SOD activity in renal tissues compared to PTU group. Serum creatinine and blood urea nitrogen (BUN) in PTU group was higher than in comparison with the control group. 400 mg/kg decreased creatinine, but both 200 and 400 mg/kg improved BUN concentration compared to PTU group.
The results of this study demonstrate that the hydroalcoholic extract of NS has a protective effect on the renal tissue oxidative damage associated with PTU-induced hypothyroidism during neonatal and juvenile growth in rats.
我们研究了黑种草水醇提取物（NS）对大鼠新生儿和幼年生长期间丙硫氧嘧啶（PTU）诱导的甲状腺功能减退相关肾组织氧化损伤的影响。
将怀孕大鼠分为五组，分别为：1）对照组；2）丙硫氧嘧啶（PTU）组；3）PTU-NS100组；4）PTU-NS200组和5）PTU-NS400组。除对照组外，所有母亲在哺乳期饮用含0.005% PTU的水。除PTU外，第3 - 5组的母亲分别接受100、200和400 mg/kg的NS提取物。哺乳期后，后代在生命的前8周继续接受相同的实验处理。每组随机选取10只雄性后代，采集血样并取出肾脏组织。
PTU组血清甲状腺素浓度低于对照组，提取物可使其改善。与对照组相比，PTU增加了肾丙二醛（MDA）含量，同时降低了总硫醇浓度以及过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性。与PTU组相比，给予200和400 mg/kg的NS提取物可降低MDA水平，增加总硫醇含量，且400 mg/kg可增加肾组织中的CAT和SOD活性。PTU组血清肌酐和血尿素氮（BUN）高于对照组。与PTU组相比，400 mg/kg可降低肌酐，200和400 mg/kg均可改善BUN浓度。
本研究结果表明，NS水醇提取物对大鼠新生儿和幼年生长期间PTU诱导的甲状腺功能减退相关肾组织氧化损伤具有保护作用。
