Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Biosens Bioelectron. 2017 Nov 15;97:253-259. doi: 10.1016/j.bios.2017.05.044.
A novel ultrasensitive label-free immunosensor based on BiOBr/AgS composite with high visible-light photoelectrochemical activity was prepared for the detection of insulin. After BiOBr was modified by thioglycolic acid, AgS nanoparticles were grown in-situ on the surface of BiOBr hierarchical microspheres to first form novel BiOBr/AgS composite. When ascorbic acid (AA) was used as an efficient electron donor for scavenging photo-generated holes, BiOBr/AgS composite material showed excellent photoelectrochemical activity. In order to immobilize insulin antibody, adhesive polydopamine (PDA) film formed by self-polymerization of dopamine was fabricated onto BiOBr/AgS modified electrode. Moreover, PDA film could further enhance the visible light absorption of BiOBr/AgS. When the solutions of 0.08molLAgNO and 0.1molL AA were selected respectively during fabrication and detection process of this sensor, the best photocurrent singles were obtained. Under the optimum experimental condition, the specific binding between insulin and antibody resulted in a decrease in photocurrent intensity and the intensity decreased linearly with the logarithm of insulin concentration in the range of 0.001-20ngmL with a detection limit of 0.2pgmL. The photoelectrochemical sensor ITO/BiOBr/AgS/PDA/anti-Insulin/BSA/Insulin revealed facile preparation, high sensitivity, and acceptable reproducibility, which may have practical applications in the biosensor, clinical diagnosis of cancers, photocatalysis, and other related fields.
一种基于具有高光电流光电化学活性的 BiOBr/AgS 复合材料的新型超灵敏无标记免疫传感器，用于胰岛素的检测。在 BiOBr 经巯基乙酸修饰后，AgS 纳米颗粒原位生长在 BiOBr 分级微球表面上，首先形成新型 BiOBr/AgS 复合材料。当抗坏血酸（AA）用作光生空穴的有效电子供体以清除空穴时，BiOBr/AgS 复合材料表现出优异的光电化学活性。为了固定胰岛素抗体，通过多巴胺自聚合形成的粘性聚多巴胺（PDA）薄膜被制备在 BiOBr/AgS 修饰电极上。此外，PDA 薄膜可以进一步增强 BiOBr/AgS 的可见光吸收。当在传感器的制造和检测过程中分别选择 0.08molLAgNO 和 0.1molL AA 的溶液时，获得了最佳的光电流单值。在最佳实验条件下，胰岛素和抗体之间的特异性结合导致光电流强度降低，光电流强度随胰岛素浓度的对数在 0.001-20ngmL 范围内呈线性下降，检测限为 0.2pgmL。ITO/BiOBr/AgS/PDA/anti-Insulin/BSA/Insulin 光电化学传感器具有制备简便、灵敏度高、重现性好等优点，在生物传感器、癌症临床诊断、光催化等相关领域具有实际应用前景。
