Işcan M
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1985;81(1):155-8.
In vitro addition of cadmium chloride (CdCl2) to a reaction mixture decreased the liver microsomal cytochrome P-450 level of the male guinea-pig. In contrast nickel chloride (NiCl2) did not cause any alteration in the cytochrome P-450 level. Neither CdCl2 nor NiCl2 produced any activity changes in the liver microsomal NADPH-cytochrome c reductase. When the liver microsomes were preincubated in the presence of CdCl2, metal-induced reduction increased as the time of preincubation progressed and attained its maximum reduction level at about 15 min. In the case of NiCl2, the maximal reduction level was attained at about 5 min. However, no changes were observed by metals in liver microsomal NADPH-cytochrome c reductase activity as the time of preincubation progressed. After preincubation, the reduction of cytochrome P-450 achieved by CdCl2 was stronger than that obtained by NiCl2 as well as than that obtained by CdCl2 without preincubation.
在反应混合物中体外添加氯化镉（CdCl₂）会降低雄性豚鼠肝脏微粒体细胞色素P - 450水平。相比之下，氯化镍（NiCl₂）不会引起细胞色素P - 450水平的任何改变。CdCl₂和NiCl₂均未使肝脏微粒体NADPH - 细胞色素c还原酶产生任何活性变化。当肝脏微粒体在CdCl₂存在下进行预孵育时，随着预孵育时间的延长，金属诱导的还原作用增强，并在约15分钟时达到最大还原水平。在NiCl₂的情况下，最大还原水平在约5分钟时达到。然而，随着预孵育时间的延长，未观察到金属对肝脏微粒体NADPH - 细胞色素c还原酶活性有任何变化。预孵育后，CdCl₂实现的细胞色素P - 450还原作用比NiCl₂以及未进行预孵育的CdCl₂所实现的更强。
