Sharma Pragya, Katoch Kiran, Chandra Shilpi, Chauhan Devendra Singh, Sharma Vishnu Dutt, Couvin David, Rastogi Nalin, Katoch Vishwa Mohan
Department of Microbiology and Molecular Biology, National JALMA Institute for Leprosy and Other Mycobacterial Diseases (ICMR), Tajganj, Agra 282004, India.
Medical Unit, National JALMA Institute for Leprosy and Other Mycobacterial Diseases (ICMR), Tajganj, Agra 282004, India; Model Rural Health Research Unit, Ghatampur, India.
Tuberculosis (Edinb). 2017 Jul;105:60-72. doi: 10.1016/j.tube.2017.04.003. Epub 2017 Apr 10.
This study was carried out to characterize Mycobacterium tuberculosis population in Ghatampur, Kanpur, North India, by spoligotyping and Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats (MIRU-VNTRs) typing. A total of 335 isolates were genotyped by spoligotyping and Central Asian (CAS) sub-lineage was the most prevalent, comprising 59.1% of all isolates. Other lineages were: East-African Indian (EAI) (19.10%), T (5.07%), Beijing (3.28%), Manu (2.98%), X (2.68%), S (0.89%), H3 (0.59%), Ural (0.59%), LAM 9 (0.29%) and unknown (5.37%). This data was compared with 8444 clinical isolates from other parts of India and neighboring countries. Thanks to interrogation of the SITVIT2 database, which shows that China is unique in having a predominance of Beijing lineage; Iran in having an almost equal proportion of Ural and CAS lineages; while the rest of the Middle-East and Indian subcontinent shows a gradient of CAS lineage predominating in the north of tropic of cancer, and the ancestral EAI lineage in South India and South-East Asia. Additionally, 12 loci MIRU-VNTR typing efficiently discriminated 13 spoligotype-defined clusters into 92 patterns; 53 isolates showed >70% homology. It was observed that Beijing lineage strains were more frequently associated with MDR strains (p-value = 0.001). A multi-step application of combination of spoligotyping and MIRU-VNTR typing for analyzing the molecular epidemiology of TB may provide a better means of fingerprinting and studying transmission dynamics.
本研究旨在通过间隔寡核苷酸分型（spoligotyping）和分枝杆菌插入重复单位-可变数目串联重复序列（MIRU-VNTRs）分型，对印度北部坎普尔加坦布尔的结核分枝杆菌群体进行特征分析。通过间隔寡核苷酸分型对总共335株分离株进行基因分型，中亚（CAS）亚谱系最为常见，占所有分离株的59.1%。其他谱系包括：东非印度（EAI）（19.10%）、T（5.07%）、北京（3.28%）、马努（2.98%）、X（2.68%）、S（0.89%）、H3（0.59%）、乌拉尔（0.59%）、LAM 9（0.29%）以及未知（5.37%）。该数据与来自印度其他地区和邻国的8444株临床分离株进行了比较。通过查询SITVIT2数据库可知，中国的独特之处在于北京谱系占主导地位；伊朗的乌拉尔谱系和CAS谱系比例几乎相等；而中东其他地区和印度次大陆显示出一种梯度分布，即北回归线以北以CAS谱系为主，印度南部和东南亚以祖传的EAI谱系为主。此外，12个位点的MIRU-VNTR分型有效地将13个由间隔寡核苷酸分型定义的簇区分成92种模式；53株分离株显示出>70%的同源性。观察发现，北京谱系菌株与耐多药菌株的关联更为频繁（p值 = 0.001）。间隔寡核苷酸分型和MIRU-VNTR分型相结合的多步骤应用，用于分析结核病的分子流行病学，可能会提供一种更好的指纹识别和研究传播动态的方法。
