Lin-Ling Ju, Lin Chen, Yi-Fan Wang, Ru-Jian Lu, Zhao-Lian Bian, Jian-Guo Shao, Nantong Institute of Liver Diseases, Nantong Third People's Hospital, Nantong University, Nantong 226006, Jiangsu Province, China.
World J Gastroenterol. 2017 May 28;23(20):3675-3683. doi: 10.3748/wjg.v23.i20.3675.
To investigate the role of nuclear division cycle (NDC)80 in human hepatocellular carcinogenesis.
gene expression was analyzed by real-time reverse transcription polymerase chain reaction in 47 paired hepatocellular carcinoma (HCC) and adjacent tissues. The HCC cell line SMMC-7721 was transfected with lentivirus to silence endogenous gene expression, which was confirmed by real-time polymerase chain reaction and western blotting. The effects of NDC80 silencing on SMMC-7721 cell proliferation were evaluated by Cellomics ArrayScan VTI imaging. Cell cycle analysis and apoptosis were detected with flow cytometry. Colony formation was assessed by fluorescence microscopy.
NDC80 expression levels in HCC tissues were significantly higher than those in the adjacent tissues. Functional studies demonstrated that NDC80 silencing significantly reduced SMMC-7721 cell proliferation and colony formation. Knockdown of NDC80 resulted in increased apoptosis and cell cycle arrest at S-phase. NDC80 contributed to HCC progression by reducing apoptosis and overcoming cell cycle arrest.
Elevated expression of NDC80 may play a role in promoting the development of HCC.
研究核分裂周期（NDC）80 在人肝细胞癌发生中的作用。
采用实时逆转录聚合酶链反应分析 47 对肝癌（HCC）及其相邻组织中的基因表达。用慢病毒转染 HCC 细胞系 SMMC-7721 以沉默内源性基因表达，通过实时聚合酶链反应和 Western blot 进行验证。用 Cellomics ArrayScan VTI 成像评估 NDC80 沉默对 SMMC-7721 细胞增殖的影响。用流式细胞术检测细胞周期分析和细胞凋亡。用荧光显微镜评估集落形成。
NDC80 在 HCC 组织中的表达水平明显高于相邻组织。功能研究表明，NDC80 沉默显著降低了 SMMC-7721 细胞的增殖和集落形成。NDC80 的敲低导致 S 期细胞凋亡增加和细胞周期阻滞。NDC80 通过减少细胞凋亡和克服细胞周期阻滞促进 HCC 的进展。
NDC80 的高表达可能在促进 HCC 的发展中起作用。
