靶细胞对免疫毒素的敏感性：细胞表面抗原的可能作用。
Sensitivity of target cells to immunotoxins: possible role of cell-surface antigens.
作者信息
Colombatti M, Bron C
出版信息
Immunology. 1985 Jun;55(2):331-8.
In this paper, we describe the activity of conjugates constituted of monoclonal antibodies chemically coupled to ricin and the ricin A-chain-like toxin gelonin. Several antibody-toxin conjugates (immunotoxins) were assayed against a Thy 1.2+ and Lyt 2.2+ T-cell line. Anti-Thy 1.2-gelonin and anti-Lyt 2.2-ricin immunotoxins (IT) had a good cytotoxic activity against target cells, whereas anti-Lyt 2.2-gelonin showed weak or no cytotoxicity. An anti-Lyt 2.2-gelonin IT displayed detectable cytotoxicity only when cells were pretreated with ammonium chloride. IT binding activity was very similar for anti-Thy 1.2 and anti-Lyt 2.2 IT, and surface antigen density was of the same order of magnitude for Thy 1.2 and Lyt 2.2 antigens. Our results suggest that intrinsic properties of the surface target antigen are able to influence the IT cytotoxic efficacy.
在本文中，我们描述了由化学偶联至蓖麻毒素和蓖麻毒素A链样毒素相思豆毒蛋白的单克隆抗体构成的偶联物的活性。针对一个Thy 1.2+和Lyt 2.2+ T细胞系检测了几种抗体-毒素偶联物（免疫毒素）。抗Thy 1.2-相思豆毒蛋白和抗Lyt 2.2-蓖麻毒素免疫毒素（IT）对靶细胞具有良好的细胞毒性活性，而抗Lyt 2.2-相思豆毒蛋白显示出微弱的细胞毒性或无细胞毒性。仅当细胞用氯化铵预处理时，一种抗Lyt 2.2-相思豆毒蛋白IT才显示出可检测到的细胞毒性。抗Thy 1.2和抗Lyt 2.2 IT的IT结合活性非常相似，并且Thy 1.2和Lyt 2.2抗原的表面抗原密度处于相同的数量级。我们的结果表明，表面靶抗原的内在特性能够影响IT的细胞毒性效力。
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