[Numb和P53蛋白在上皮性卵巢癌中的表达及临床意义]

[Expression and Clinical Significance of Numb and P53 Proteins in Epithelial Ovarian Carcinoma].

作者信息

Xu Min, He Hong-Ju, Zhang Huan, Wang Ao, Chen Jie

机构信息

Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2017 Jan;48(1):76-80.

PMID:28612563

Abstract

OBJECTIVES

To investigate the expression and clinical significance of Numb and P53 proteins in epithelial ovarian carcinoma.

METHODS

We co-tested the expressions of Numb and P53 proteins in epithelial ovarian tissues by immunohistochemistry, including 20 cases of normal tissues, 29 cases of benign tumors, 35 cases of borderline ovarian tumors and 87 cases of epithelial ovarian carcinoma. Their clinical significance was analyzed.

RESULTS

The positive expression rates of Numb protein in normal ovarian tissues, benign ovarian tumors, borderline ovarian tumors and epithelial ovarian carcinoma were 5%, 13.8%, 51.4% and 70.1%, respectively, and with the rising trend and significantly difference among four groups ( <0.05). The expression of Numb protein had significant positive correlation with stage and lymph node metastasis ( <0.05), while Numb expression had no correlation with age, pathological type or histological grade ( >0.05). The positive expression rates of P53 protein in normal ovarian tissues, benign ovarian tumors, borderline ovarian tumors and epithelial ovarian carcinoma were 0%, 3.4%, 28.6% and 63.2%, respectively, which had a gradually rising trend with significantly difference among four groups ( <0.05). The expression of P53 protein in borderline ovarian tumors was significantly higher than those in normal ovarian tissues and benign ovarian tumors ( <0.05). The expression of P53 protein in epithelial ovarian carcinoma group was significantly higher than those in the rest three groups ( <0.05). Statistical significance was found between the expression of P53 protein and histological type, differentiation, stage, lymph node metastasis ( <0.05); But Numb expression had no correlation with age ( >0.05). The expression of Numb protein was positive correlated with the expression of P53 protein in borderline ovarian tumors and epithelial ovarian carcinoma ( =0.488, <0.05; =0.231, <0.05).

CONCLUSIONS

Co-testing Numb and P53 protein might be helpful for the diagnosis and evaluation of prognosis of epithelial ovarian carcinoma and two proteins might work together in the development process of epithelial ovarian carcinoma.

摘要

目的

探讨Numb和P53蛋白在上皮性卵巢癌中的表达及临床意义。

方法

采用免疫组织化学方法联合检测20例正常卵巢组织、29例良性肿瘤、35例卵巢交界性肿瘤及87例上皮性卵巢癌组织中Numb和P53蛋白的表达，并分析其临床意义。

结果

Numb蛋白在正常卵巢组织、卵巢良性肿瘤、卵巢交界性肿瘤及上皮性卵巢癌中的阳性表达率分别为5%、13.8%、51.4%和70.1%，呈上升趋势，四组间差异有统计学意义（<0.05）。Numb蛋白表达与分期及淋巴结转移呈显著正相关（<0.05），而与年龄、病理类型或组织学分级无关（>0.05）。P53蛋白在正常卵巢组织、卵巢良性肿瘤、卵巢交界性肿瘤及上皮性卵巢癌中的阳性表达率分别为0%、3.4%、28.6%和63.2%，呈逐渐上升趋势，四组间差异有统计学意义（<0.05）。卵巢交界性肿瘤中P53蛋白表达显著高于正常卵巢组织和卵巢良性肿瘤（<0.05）。上皮性卵巢癌组P53蛋白表达显著高于其余三组（<0.05）。P53蛋白表达与组织学类型、分化程度、分期、淋巴结转移之间差异有统计学意义（<0.05）；但Numb表达与年龄无关（>0.05）。在卵巢交界性肿瘤和上皮性卵巢癌中，Numb蛋白表达与P53蛋白表达呈正相关（=0.488，<0.05；=0.231，<0.05）。