Incorporation and toxicity of 32P-orthophosphate and occurrence of polyphosphate in Entamoeba trophozoites.
作者信息
López-Revilla R, Gómez-Domínguez R
出版信息
J Protozool. 1985 May;32(2):353-5. doi: 10.1111/j.1550-7408.1985.tb03065.x.
Abstract
We explored the requirements of inorganic phosphate (Pi), the incorporation of 32P-orthophosphate (32Pi), and the occurrence of inorganic polyphosphate (polyP) in axenic Entamoeba cultures. Maximal population densities and growth rates of Entamoeba histolytica trophozoites were attained in complete TP-S-1 medium. As 32Pi concentration was increased in the medium, its own incorporation and the culture growth rate were progressively inhibited, especially in Pi-deficient medium. PolyP grains were found in the cytoplasm and occasionally in the nuclear membrane of E. histolytica-like, E. invadens, and E. moshkovskii trophozoites.
摘要
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