Pereira V R Z B, Wolf J M, Stumm G Z, Boeira T R, Galvan J, Simon D, Lunge V R
, , Brasil.
Prefeitura Municipal de Caxias do Sul, Serviço Municipal de Infectologia, , Brasil.
Genet Mol Res. 2017 May 31;16(2):gmr-16-02-gmr.16029565. doi: 10.4238/gmr16029565.
Hepatitis B virus (HBV) infection is a serious public health problem worldwide. The progression of the disease depends on several host and viral factors and may result in fulminant hepatitis (very rare), acute hepatitis with spontaneous clearance, and chronic hepatitis B infection. Previous studies demonstrated that variations in the human leukocyte antigen (HLA) class II (HLA-DPB1 and HLA-DQB2 genes) are related to the chronic HBV infection. This study aimed to investigate the association of two single nucleotide polymorphism (SNPs), one in the HLA-DPB1 (rs9277535) and one in the HLA-DQB2 (rs7453920), with chronic hepatitis B infection in a southern Brazilian sample. This case-control study included 260 HBV patients attended in a Specialized Center for Health in Caxias do Sul (Brazil) between 2014 and 2016. The same number of controls (matching for age, gender, and ethnicity) was obtained in a University Hospital in the same city and period. Blood samples were collected and genomic DNA was extracted. Genotyping were performed by real-time Taqman PCR method. Odds ratios with 95% confidence intervals and significance level of 5% (P < 0.05) were calculated. Allele frequencies in the SNP rs9277535 were 72.6% for A and 27.4% for G nucleotides in cases and 75.0% for A and 25.0% for G in controls. Allele frequencies in the SNP rs7453920 were of 25.7% for A and 74.3% for G in cases and 28.8% for A and 71.2% for G in controls. No statistically significant association was found between both SNPs and chronic hepatitis B (P > 0.05).
乙型肝炎病毒（HBV）感染是一个全球性的严重公共卫生问题。疾病的进展取决于多种宿主和病毒因素，可能导致暴发性肝炎（非常罕见）、急性肝炎伴自发清除，以及慢性乙型肝炎感染。先前的研究表明，人类白细胞抗原（HLA）II类（HLA - DPB1和HLA - DQB2基因）的变异与慢性HBV感染有关。本研究旨在调查巴西南部样本中HLA - DPB1（rs9277535）和HLA - DQB2（rs7453920）两个单核苷酸多态性（SNP）与慢性乙型肝炎感染的关联。这项病例对照研究纳入了2014年至2016年期间在巴西南里奥格兰德州卡西亚斯杜尔市一家专门的健康中心就诊的260例HBV患者。在同一城市和时期的一家大学医院选取了相同数量的对照（按年龄、性别和种族匹配）。采集血样并提取基因组DNA。通过实时Taqman PCR方法进行基因分型。计算比值比及95%置信区间，显著性水平为5%（P < 0.05）。病例组中SNP rs9277535的等位基因频率为A核苷酸72.6%、G核苷酸27.4%，对照组中A为75.0%、G为25.0%。病例组中SNP rs7453920的等位基因频率为A 25.7%、G 74.3%，对照组中A为28.8%、G为71.2%。未发现这两个SNP与慢性乙型肝炎之间存在统计学显著关联（P > 0.05）。
