Pereira-Defilippi L, Pereira E M, Silva F M, Moro G V
Departamento de Produção Vegetal, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual de São Paulo "Júlio de Mesquita Filho", Jaboticabal, SP, Brasil
Departamento de Produção Vegetal, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual de São Paulo "Júlio de Mesquita Filho", Jaboticabal, SP, Brasil.
Genet Mol Res. 2017 May 31;16(2):gmr-16-02-gmr.16029682. doi: 10.4238/gmr16029682.
The relative quantitative real-time expression of two expressed sequence tags (ESTs) codifying for key enzymes in nitrogen metabolism in maize, nitrate reductase (ZmNR), and glutamine synthetase (ZmGln1-3) was performed for genotypes inoculated with Azospirillum brasilense. Two commercial single-cross hybrids (AG7098 and 2B707) and two experimental synthetic varieties (V2 and V4) were raised under controlled greenhouse conditions, in six treatment groups corresponding to different forms of inoculation and different levels of nitrogen application by top-dressing. The genotypes presented distinct responses to inoculation with A. brasilense. Increases in the expression of ZmNR were observed for the hybrids, while V4 only displayed a greater level of expression when the plants received nitrogenous fertilization by top-dressing and there was no inoculation. The expression of the ZmGln1-3EST was induced by A. brasilense in the hybrids and the variety V4. In contrast, the variety V2 did not respond to inoculation.
对接种巴西固氮螺菌的玉米基因型，进行了编码氮代谢关键酶的两个表达序列标签（EST），即硝酸还原酶（ZmNR）和谷氨酰胺合成酶（ZmGln1-3）的相对定量实时表达分析。在可控温室条件下，将两个商业单交种（AG7098和2B707）和两个实验合成品种（V2和V4）分为六个处理组进行培育，这些处理组对应不同的接种形式和不同的追肥施氮水平。基因型对巴西固氮螺菌接种表现出不同反应。杂交种中观察到ZmNR表达增加，而V4仅在植株通过追肥施氮肥且未接种时表现出更高的表达水平。ZmGln1-3 EST的表达在杂交种和V4品种中由巴西固氮螺菌诱导。相反，V2品种对接种无反应。
