Departamento de Ciencias Químico-Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez , Anillo envolvente del PRONAF y Estocolmo s/n, Ciudad Juárez, Chihuahua 32310, México.
Coordinación de Tecnología de Alimentos de Origen Vegetal, Centro de Investigación en Alimentación y Desarrollo , Carretera a la Victoria Km 0.6, Hermosillo, Sonora CP 8300, México.
J Agric Food Chem. 2017 Jul 5;65(26):5244-5254. doi: 10.1021/acs.jafc.7b01702. Epub 2017 Jun 26.
The effect of four elicitors on phytochemical content in two varieties of lettuce was evaluated. The best preharvest day for application of each elicitor was chosen. Solutions of arachidonic acid (AA), salicylic acid (SA), methyl jasmonate (MJ), and Harpin protein (HP) were applied by foliar aspersion on lettuce leaves while cultivating under hydroponic conditions. Application of elicitors was done at 15, 7, 5, 3, or 1 day before harvest. Green lettuce showed the highest increase in phytochemical content when elicitors (AA, SA, and HP) were applied on day 7 before harvest. Similarly, antioxidant activity rose in all treatments on day 7. In red lettuce, the highest content of bioactive molecules occurred in samples treated on day 15. AA, SA, and HP were the elicitors with the highest effect on phytochemical content for both varieties, mainly on polyphenol content. Antioxidant activity also increased in response to elicitation. HPLC-MS showed an increase in the content of phenolic acids in green and red lettuce, especially after elicitation with SA, suggesting activation of the caffeic acid pathway due to elicitation.
四种诱导剂对两种生菜品种中植物化学物质含量的影响进行了评估。选择了每种诱导剂的最佳收获前施用天数。在水培条件下培养生菜叶片时，通过叶面喷雾施用花生四烯酸（AA）、水杨酸（SA）、茉莉酸甲酯（MJ）和 Harpin 蛋白（HP）溶液。在收获前 15、7、5、3 或 1 天施用诱导剂。当在收获前 7 天施用诱导剂（AA、SA 和 HP）时，绿色生菜的植物化学物质含量增加最高。同样，在第 7 天所有处理的抗氧化活性都增加了。在红色生菜中，生物活性分子含量最高的样品出现在处理 15 天后。AA、SA 和 HP 是两种品种中对植物化学物质含量影响最大的诱导剂，主要影响多酚含量。抗氧化活性也因诱导而增加。HPLC-MS 显示绿色和红色生菜中酚酸含量增加，尤其是用 SA 诱导后，表明由于诱导作用激活了咖啡酸途径。
