Beijing Research Center for Agricultural Standards and Testing , No. 9 Middle Road of Shuguanghuayuan, Haidian District, Beijing 100097, China.
Risk Assessment Laboratory for Agro-products (Beijing), Ministry of Agriculture , No. 9 Middle Road of Shuguanghuayuan, Haidian District, Beijing 100097, China.
J Agric Food Chem. 2017 Jul 5;65(26):5413-5420. doi: 10.1021/acs.jafc.7b01409. Epub 2017 Jun 23.
Early and late ripening sweet cherries were characterized for phenolic acids, and also their antioxidant capacity and potential antifungal effects were investigated. Free, conjugated, and bound phenolics were identified and quantified using ultra performance liquid chromatography-tandem mass spectrometry. Our results indicated that the early ripening cultivars contained higher free phenolic acids, which was positively related to remarkable antioxidant properties and the inhibition effects on Alternaria alternata and tenuazonic acid (TeA) accumulation. However, conjugated phenolics of the late ripening cultivars, mainly including caffeic, 2,3,4-trihydroxybenzoic, p-coumaric, and pyrocatechuic acids, achieved the highest antifungal effects and almost completely inhibited the A. alternata and TeA production. 2,2-Diphenyl-1-picrylhydrazyl testing and ferric ion reducing antioxidant power assay showed strong positive correlation with total phenolics and specific phenolics such as free epicatechin and conjugated 2,3,4-trihydroxybenzoic acids and also with antifungal activity. Results from this study provide further insights into the health-promoting phenolic compounds in sweet cherries.
本研究对早、晚熟甜樱桃的酚酸组成进行了分析，并对其抗氧化能力和潜在的抗真菌作用进行了研究。采用超高效液相色谱-串联质谱法对游离、结合和结合酚进行了鉴定和定量。结果表明，早熟品种含有较高的游离酚酸，这与其显著的抗氧化特性以及对链格孢菌和棒曲霉素（TeA）积累的抑制作用呈正相关。然而，晚熟品种的结合酚酸，主要包括咖啡酸、2,3,4-三羟基苯甲酸、对香豆酸和焦儿茶酸，具有最高的抗真菌作用，几乎完全抑制了链格孢菌和 TeA 的产生。2,2-二苯基-1-苦基肼（DPPH）试验和铁离子还原抗氧化能力（FRAP） assay 与总酚和特定酚类物质（如游离表儿茶素和结合 2,3,4-三羟基苯甲酸）以及抗真菌活性呈强正相关。本研究结果为甜樱桃中具有促进健康的酚类化合物提供了进一步的认识。
