Wang X, Zhang C, Yang X D, Li B D, Zang D D, Yang P, Zhang J X, Zhu Q X
Department of Occupational and Environmental Health, School of Public Health, Anhui Medical University, Hefei 230032, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2017 Apr 20;35(4):246-250. doi: 10.3760/cma.j.issn.1001-9391.2017.04.002.
To explore the expression of CD55 in liver tissue of trichloroethylene-sensitized mice and discuss the role of CD55 in the liver immune injury of trichloroethylene-sensitized mice. 6-8 weeks specific pathogen free female BALB/c were randomly divided into blank control group, solvent control group and TCE treatment group to establish BALB/c mice sensitized model. According to mouse skin sensitization reaction score, TCE treatment mice were divided into sensitized and non-sensitized group at 24 h after the last challenge. At 48 h after the last challenge, the blood and aseptic livers were collected. The level of serum ALT was tested by automatic biochemical analyzer and pathology of the liver was observed. C5b-9 deposition was studied by immunohistochemistry (IHC) . CD55 protein expression level in liver tissue was studied by immunohistochemistry and Western blotting. The expression of CD55 mRNA in liver tissue was detected by qRT-PCR. Liver function test result showed level of serum ALT in TCE sensitized group was significantly higher than solvent control group and TCE non-sensitized group (<0.05) . There was ballooning degeneration and necrosis of liver cells in TCE sensitized group. IHC demonstrated that TCE sensitized group had obviously increased content of C5b-9 but had reduced content of CD55 compared with solvent control group and TCE non-sensitized group (<0.05) . Western blotting also showed that TCE sensitized group had lower expression of CD55 than solvent control group and TCE non-sensitized group (<0.05) . qRT-PCR showed that CD55 mRNA expression level in liver tissue of TCE sensitized group was apparently lower than solvent control group and TCE non-sensitized group (<0.05) . Complement activation may be involved in TCE-induced liver injury, and the expression change of complement regulatory protein CD55 may play essential role in the process.
探讨三氯乙烯致敏小鼠肝组织中CD55的表达，探讨CD55在三氯乙烯致敏小鼠肝脏免疫损伤中的作用。将6-8周龄无特定病原体的雌性BALB/c小鼠随机分为空白对照组、溶剂对照组和三氯乙烯处理组，建立BALB/c小鼠致敏模型。根据小鼠皮肤致敏反应评分，在末次激发后24小时将三氯乙烯处理的小鼠分为致敏组和非致敏组。在末次激发后48小时，采集血液和无菌肝脏。用自动生化分析仪检测血清ALT水平，并观察肝脏病理。通过免疫组织化学（IHC）研究C5b-9沉积。通过免疫组织化学和蛋白质印迹法研究肝组织中CD55蛋白表达水平。用qRT-PCR检测肝组织中CD55 mRNA的表达。肝功能检测结果显示，三氯乙烯致敏组血清ALT水平显著高于溶剂对照组和三氯乙烯非致敏组（<0.05）。三氯乙烯致敏组肝细胞有气球样变性和坏死。免疫组织化学显示，与溶剂对照组和三氯乙烯非致敏组相比，三氯乙烯致敏组C5b-9含量明显增加，但CD55含量降低（<0.05）。蛋白质印迹法也显示，三氯乙烯致敏组CD55表达低于溶剂对照组和三氯乙烯非致敏组（<0.05）。qRT-PCR显示，三氯乙烯致敏组肝组织中CD55 mRNA表达水平明显低于溶剂对照组和三氯乙烯非致敏组（<0.05）。补体激活可能参与三氯乙烯诱导的肝损伤，补体调节蛋白CD55的表达变化可能在该过程中起重要作用。
