Williams G A, Kukreja S C, Longley R S, Bowser E N, Hargis G K, Vora N M, Henderson W J
Metabolism. 1985 Jul;34(7):612-5. doi: 10.1016/0026-0495(85)90086-1.
This study evaluated the effect of parasympathetic agonists and antagonists on immunoreactive (i) PTH secretion in vitro and on serum iPTH in vivo in rats. In in vitro studies pilocarpine or bethanechol significantly inhibited PTH secretion. This inhibition was blocked by the simultaneous addition of atropine to the incubation medium. In in vivo studies, the cholinergic agonists pilocarpine and bethanechol and the cholinergic antagonist atropine were administered to rats by IV infusion. Blood was obtained before and again after two hours of infusion for analysis of iPTH. Pilocarpine or bethanechol significantly decreased serum iPTH. This inhibition by either agent was blocked by the simultaneous administration of atropine. Administration of atropine alone significantly increased serum iPTH above baseline. This stimulation of basal serum iPTH by parasympathetic blockade suggests that even basal PTH secretion may be influenced by endogenous parasympathetic tone. Therefore, the following conclusions were reached: (1) parasympathetic influences inhibit PTH secretion, and (2) endogenous parasympathetic tone may be an inhibitory modulator of basal secretion of PTH.
本研究评估了副交感神经激动剂和拮抗剂对大鼠体外免疫反应性（i）甲状旁腺激素（PTH）分泌及体内血清iPTH的影响。在体外研究中，毛果芸香碱或氨甲酰甲胆碱显著抑制PTH分泌。向孵育培养基中同时添加阿托品可阻断这种抑制作用。在体内研究中，通过静脉输注向大鼠给予胆碱能激动剂毛果芸香碱和氨甲酰甲胆碱以及胆碱能拮抗剂阿托品。在输注前及输注两小时后采集血液以分析iPTH。毛果芸香碱或氨甲酰甲胆碱显著降低血清iPTH。两种药物的这种抑制作用均被同时给予阿托品所阻断。单独给予阿托品显著使血清iPTH高于基线水平。副交感神经阻滞对基础血清iPTH的这种刺激表明，即使基础PTH分泌也可能受内源性副交感神经张力影响。因此，得出以下结论：（1）副交感神经影响抑制PTH分泌，（2）内源性副交感神经张力可能是PTH基础分泌的一种抑制性调节因子。
