Herold Christian, Engeli Stefan, Beckmann Bibiana, Vogt Peter M, Rennekampff Hans-Oliver
Department of Plastic and Aesthetic Surgery, Hand Surgery, Sana Klinikum Hameln Pyrmont, Hameln, Germany.
Institute of Clinical Pharmacology, Hannover Medical School, Hannover, Germany.
Indian J Plast Surg. 2017 Jan-Apr;50(1):56-63. doi: 10.4103/ijps.IJPS_103_16.
In addition to its use as a volume filler, fat grafting may have a potential role in wound healing based on the concentration of growth factors in the lipoaspirate. In this study, we compare the quantitative and qualitative concentration of the various growth factors and adipokines using the Shippert or the Coleman techniques to prepare the lipoaspirate.
We measured leptin, adiponectin and the growth factors, i.e., acidic fibroblast growth factor (aFGF), basic FGF (bFGF), keratinocyte growth factor (KGF), bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) by ELISA in solid and liquid fractions obtained with both techniques in human fat obtained with Coleman technique and Shippert technique.
All of these peptides, except BMP-2, were detected in relevant quantities in the solid fraction. The Coleman but not the Shippert technique resulted in statistically higher adiponectin concentrations in the solid tissue fraction. The other four growth factors occurred in significantly higher concentrations in the solid fractions compared to the liquid fractions, independent of the processing technique.
In summary, we demonstrated that KGF, aFGF, bFGF and VEGF, as well as leptin and adiponectin, are contained in fat suspensions obtained by liposuction and in the supernatant. Only the concentration of adiponectin was in the range reported to contribute to wound healing.
除了用作容量填充剂外，基于脂肪抽吸物中生长因子的浓度，脂肪移植在伤口愈合中可能具有潜在作用。在本研究中，我们使用希珀特（Shippert）或科尔曼（Coleman）技术制备脂肪抽吸物，比较各种生长因子和脂肪因子的定量和定性浓度。
我们通过酶联免疫吸附测定法（ELISA），在采用科尔曼技术和希珀特技术获取的人体脂肪中，对用这两种技术获得的固体和液体部分中的瘦素、脂联素以及生长因子，即酸性成纤维细胞生长因子（aFGF）、碱性成纤维细胞生长因子（bFGF）、角质形成细胞生长因子（KGF）、骨形态发生蛋白-2（BMP-2）和血管内皮生长因子（VEGF）进行了测量。
除BMP-2外，所有这些肽在固体部分中均检测到了相应数量。科尔曼技术而非希珀特技术导致固体组织部分中的脂联素浓度在统计学上更高。与液体部分相比，其他四种生长因子在固体部分中的浓度显著更高，且与处理技术无关。
总之，我们证明了通过抽脂获得的脂肪悬液及其上清液中含有KGF、aFGF、bFGF和VEGF，以及瘦素和脂联素。只有脂联素的浓度处于据报道对伤口愈合有作用的范围内。
