Chopra D P, Cooney R A
Virchows Arch B Cell Pathol Incl Mol Pathol. 1985;48(4):299-315. doi: 10.1007/BF02890137.
Cytokinetic and histogenic alterations associated with the development of benzo(a)-pyrene (BP) induced epidermoid metaplasia were studied in tracheal explants derived from normal hamsters. Treatment of the explants with BP induced hyperplasia in both the basal and mucous cells. The hyperplasia of the basal cells persisted throughout the duration of the experiment whereas the hyperplasia of the mucous cells subsided between 7 and 10 days after treatment. This was accompanied by stimulation of ciliated cell differentiation and aberrant ciliogenesis which was not limited to the surface cells since some basal cells were observed differentiating into ciliated cells. Subsequently, the differentiation of basal cells into mucous cells was inhibited. Instead, the basal cells differentiated into metaplastic cells. With the progression of the lesions, the mucociliary surface layer was sloughed into the lumen due to the population pressure from the underlying actively proliferating metaplastic cells and their subsequent epidermoid differentiation. Approximately 50% of the explants exhibited focal areas of squamous metaplasia at 7 days after the treatment and extensive epidermoid metaplasia was present in approximately 90% of the explants at 10 days. These results support the hypothesis that BP induced epidermoid metaplasia of tracheal explants originates from the basal cells.
在源自正常仓鼠的气管外植体中，研究了与苯并(a)芘（BP）诱导的表皮化生发展相关的细胞动力学和组织发生改变。用BP处理外植体可诱导基底细胞和黏液细胞增生。基底细胞的增生在整个实验期间持续存在，而黏液细胞的增生在处理后7至10天消退。这伴随着纤毛细胞分化的刺激和异常的纤毛发生，这种异常不仅限于表面细胞，因为观察到一些基底细胞分化为纤毛细胞。随后，基底细胞向黏液细胞的分化受到抑制。相反，基底细胞分化为化生细胞。随着病变的进展，由于来自下方活跃增殖的化生细胞及其随后的表皮样分化的群体压力，黏液纤毛表面层脱落到管腔中。约50%的外植体在处理后7天出现局灶性鳞状化生区域，约90%的外植体在10天出现广泛的表皮样化生。这些结果支持了BP诱导气管外植体表皮样化生起源于基底细胞的假说。
