Huang Dan, Liu Hong-Hong, Yang Zhen, Qu Yun
Department of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Key Laboratory of Rehabilitation Medicine, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2017 May;48(3):378-383.
To determine the effects of non-invasive limb ischemic postconditioning (NLIP) on the behavioral performance and the expression and distribution of neural cells in rats with ischemic cortex.
Rats were randomly divided into 3 groups: sham group (=10), ischemia/reperfusion (I/R) group (=50), and NLIP group (=50). Focal cerebral ischemia was induced by intraluminal MCAO in the rats in the I/R and NLIP groups, while no suture was inserted in the sham-operated rats. NLIP (reperfusion-10 min, ischemia-10 min, ×3 cycles) was conducted immediately after reperfusion on bilateral femoral arteries by modified ischemicelastic bands. Body mass and behavioral performance of the rats were assessed at 3 d, 7 d, 14 d, 21 d and 28 d post-ischemia (=5-10). Doublecortin (DCX) and glial fibrillary acidic protein (GFAP) in the ischemic cortex were detected by immunohistochemistry.The expression of microtubule associated protein 2 (MAP2) was detected by Western blot.
The rats in the sham group showed mild body mass loss and minor neurological deficit 1-3 d post-ischemia and weakened muscle strength 1 d post-ischemia, which were alleviated gradually overtime. Compared with the sham group, neurological deficits were more obvious in the rats in the I/R and NLIP groups, which were alleviated 3-21 d post-ischemia and reached a level close to that of the sham group at 28 d post-ischemia. No significant difference was found betweenthe I/R and NLIP groups in neurologic deficit scores.NLIP significantly alleviated body mass loss 2-7 d post-ischemia (<0.05) and improved muscle strength 14-28 d post-ischemia (<0.05).Compared with the sham group, rats in the I/R and NLP groups had increased numbers of DCX and GFAP-labeled cells in the ischemic penumbra over time, increased hypotrophic cell bodies and longer and thickened dendrites, and decreased expression of MAP2 (>0.05) at 3 d post-ischemia prior to an up-regulation.Higher expression of MAP2 was found 14-21 d post-ischemia (<0.05) in the I/R group and 7-28 d post-ischemia in the NLIP group(<0.05). Significant difference in MAP2 was found between the I/R and NLIP groups at 7 d post-ischemia (<0.05).
NLIP has the potential to improve neurological outcomes and promote increase of neural cells in penumbral cortex after cerebral ischemia.
确定非侵入性肢体缺血后处理（NLIP）对缺血性皮质大鼠行为表现以及神经细胞表达和分布的影响。
将大鼠随机分为3组：假手术组（n = 10）、缺血/再灌注（I/R）组（n = 50）和NLIP组（n = 50）。I/R组和NLIP组大鼠通过大脑中动脉线栓法诱导局灶性脑缺血，假手术组大鼠不插入线栓。NLIP（再灌注10分钟，缺血10分钟，共3个循环）在再灌注后立即通过改良的缺血弹性带对双侧股动脉进行处理。在缺血后3天、7天、14天、21天和28天评估大鼠的体重和行为表现（每组n = 5 - 10）。通过免疫组织化学检测缺血皮质中的双皮质素（DCX）和胶质纤维酸性蛋白（GFAP）。通过蛋白质免疫印迹法检测微管相关蛋白2（MAP2）的表达。
假手术组大鼠在缺血后1 - 3天体重轻度下降，有轻微神经功能缺损，缺血后1天肌肉力量减弱，随着时间推移逐渐缓解。与假手术组相比，I/R组和NLIP组大鼠的神经功能缺损更明显，在缺血后3 - 21天缓解，在缺血后28天达到接近假手术组的水平。I/R组和NLIP组在神经功能缺损评分上无显著差异。NLIP显著减轻缺血后2 - 7天的体重下降（P<0.05），并改善缺血后14 - 28天的肌肉力量（P<0.05）。与假手术组相比，I/R组和NLIP组大鼠缺血半暗带中DCX和GFAP标记细胞数量随时间增加，细胞体萎缩、树突变长变粗，缺血后3天MAP2表达降低（P>0.05），之后上调。I/R组在缺血后14 - 21天MAP2表达较高（P<0.05），NLIP组在缺血后7 - 28天MAP2表达较高（P<0.05）。缺血后7天I/R组和NLIP组MAP2表达有显著差异（P<0.05）。
NLIP有改善神经功能结局并促进脑缺血后半暗带皮质神经细胞增加的潜力。
