Khot Vinita V, Yadav Dilip K, Shrestha Smeeta, Kaur Lovejeet, Sundrani Deepali P, Chavan-Gautam Preeti M, Mehendale Savita S, Chandak Giriraj R, Joshi Sadhana R
Departments of Nutritional Medicine, Interactive Research School for Health Affairs, Bharati Vidyapeeth University, Pune 411043, Maharashtra, India.
Genomic Research on Complex Diseases (GRC Group), CSIR-Centre for Cellular & Molecular Biology (CSIR-CCMB), Habsiguda, Uppal Road, Hyderabad 500007, Telangana, India.
Epigenomics. 2017 Jul;9(7):985-996. doi: 10.2217/epi-2016-0173. Epub 2017 Jun 15.
Altered maternal one-carbon metabolism influences placental DNA methylation patterns and 'programs' the fetus for noncommunicable diseases in adult life.
Levels of plasma folate, vitamin B, homocysteine, mRNA and protein levels of MTHFR and MTR enzymes in placenta were compared among women delivering preterm (n = 83) and term (n = 75). MTR promoter CpG methylation was undertaken.
MTHFR and MTR mRNA levels were higher while protein levels were lower, and MTR CpG sites were hypermethylated in the preterm group, as compared with the term group. Methylated CpG sites were negatively associated with maternal plasma vitamin B levels.
Study suggests a dysregulation of enzyme genes in remethylation arm of the one-carbon metabolism in placenta of women delivering preterm.
母体一碳代谢改变会影响胎盘DNA甲基化模式，并为胎儿成年后患非传染性疾病“编程”。
比较了早产（n = 83）和足月产（n = 75）女性胎盘组织中血浆叶酸、维生素B、同型半胱氨酸水平，以及MTHFR和MTR酶的mRNA和蛋白质水平。进行了MTR启动子CpG甲基化分析。
与足月产组相比，早产组MTHFR和MTR的mRNA水平较高，而蛋白质水平较低，且MTR的CpG位点存在高甲基化。甲基化的CpG位点与母体血浆维生素B水平呈负相关。
研究表明，早产女性胎盘组织中一碳代谢再甲基化途径的酶基因存在失调。
