Scarel-Caminaga Raquel M, Cera Flávia F, Pigossi Suzane C, Finoti Livia S, Kim Yeon J, Viana Aline C, Secolin Rodrigo, Montenegro Marcelo F, Tanus-Santos José E, Orrico Silvana R P, Cirelli Joni A
Department of Morphology, School of Dentistry at Araraquara, São Paulo State University (UNESP), Humaita St., 1680, Araraquara CEP 14801-903, São Paulo, Brazil.
Department of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University (UNESP), Humaita St., 1680, Araraquara CEP 14801-903, São Paulo, Brazil.
Int J Mol Sci. 2017 Jun 15;18(6):1128. doi: 10.3390/ijms18061128.
This study aimed to investigate whether the -1026(A>C)(rs2779249) and +2087(A>G)(2297518) polymorphisms in the gene were associated with chronic periodontitis (CP) and with salivary levels of nitrite (NO₂) and/or nitrate + nitrite (NOx). A group of 113 mixed-race patients were subjected to periodontal, genetic, and biochemical evaluations (65 CP/48 periodontally healthy subjects). DNA was extracted from oral epithelial cells and used for genotyping by polymerase chain reaction (real-time). Salivary NOx concentrations were determined using an ozone-based chemiluminescence assay. Association of CP with alleles and genotypes of the -1026(A>C) polymorphism was found (² test, = 0.0075; 0.0308), but this was not maintained after multiple logistic regression, performed to estimate the effect of covariates and polymorphisms in CP. This analysis demonstrated, after correction for multiple comparisons, that only the female gender was significantly associated with CP. Polymorphisms analyzed as haplotypes were not associated with CP. NOx levels were significantly higher in the control group of heterozygous individuals for both polymorphisms. In conclusion, the female gender was significantly associated with CP, and higher levels of salivary NOx were found in control subjects and associated with the heterozygous state of the polymorphisms, reinforcing the potential of NO metabolites as markers of periodontitis status.
本研究旨在调查该基因中的 -1026(A>C)(rs2779249) 和 +2087(A>G)(2297518) 多态性是否与慢性牙周炎 (CP) 以及唾液中亚硝酸盐 (NO₂) 和/或硝酸盐+亚硝酸盐 (NOx) 的水平相关。一组113名混血患者接受了牙周、基因和生化评估（65名CP患者/48名牙周健康受试者）。从口腔上皮细胞中提取DNA，并通过聚合酶链反应（实时）用于基因分型。使用基于臭氧的化学发光分析法测定唾液中NOx的浓度。发现CP与 -1026(A>C) 多态性的等位基因和基因型存在关联（卡方检验，P = 0.0075；0.0308），但在进行多因素逻辑回归以估计协变量和多态性对CP的影响后，这种关联未得到维持。该分析表明，在进行多重比较校正后，只有女性性别与CP显著相关。作为单倍型分析的多态性与CP无关。在两种多态性的杂合个体对照组中，NOx水平显著更高。总之，女性性别与CP显著相关，并且在对照受试者中发现唾液NOx水平更高，且与多态性的杂合状态相关，这进一步证明了NO代谢产物作为牙周炎状态标志物的潜力。
