硫醇-烯微柱列电喷雾离子化平台，用于飞摩尔级别的生物分析。

Thiol-ene micropillar array electrospray ionization platform for zeptomole level bioanalysis.

机构信息

Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, P.O. Box 56, FI-00014. and University of Helsinki, Finland.

出版信息

Analyst. 2017 Jul 10;142(14):2552-2557. doi: 10.1039/c7an00544j.

DOI:10.1039/c7an00544j

PMID:28617495

Abstract

A micropillar array electrospray ionization (μPESI) platform fabricated from thiol-enes with 56 individual polyethylene glycol coated μPESI chips for bioanalytical mass spectrometry is introduced. Bioanalysis capability is shown by measurement of a protein, a protein digest and a cell lysate sample. The thiol-ene polyethylene glycol (PEG) coated μPESI chip allows the use of a wide range of aqueous-organic solvent compositions and provides a detection limit at 60 zeptomole level (6 × 10 mol) for a peptide standard.

摘要

介绍了一种由硫醇-烯体制备的微针阵列电喷雾电离（μPESI）平台，该平台带有 56 个单独的聚乙二醇涂层 μPESI 芯片，可用于生物分析质谱。通过测量蛋白质、蛋白质消化物和细胞裂解物样品，展示了该平台的生物分析能力。硫醇-烯基聚乙二醇（PEG）涂层的 μPESI 芯片允许使用广泛的水-有机溶剂组成，并为肽标准品提供了 60 飞摩尔级（6×10-18 mol）的检测限。