Sun Q, Jiang C-W, Tan Z-H, Hou L-Y, Dong H, Liu K, Sun G, Liu Y-J, Wang Y-Q, Lu X-C, Liu H
Department of Intensive Care Unit, Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.
Eur Rev Med Pharmacol Sci. 2017 May;21(10):2385-2391.
The lung adenocarcinoma is a type of lung cancer. This research is to investigate the effects of miR-222 on the proliferation, migration and invasion of the lung adenocarcinoma cells.
At the beginning, MiR-222 and the controls were transfected to the lung adenocarcinoma cell line A549 for CCK-8 proliferation, transwell migration and Matrigel invasion, and then observed the effect of miR-222 on the proliferation, migration and invasion of lung adenocarcinoma cells. The miR-222 target was regulated by ETS1 downwards to participate in the regulation of the process by using the luciferase reporter assay, the Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and the Western blotting.
According to CCK-8 proliferation assay, the Transwell migration and the Matrigel invasion assay, it discovered that MiR-222 can promote the proliferation, migration and invasion of the lung adenocarcinoma cells. Luciferase reporter assay, RT-qPCR and Western blot assay showed that miR-222 could regulate the expression of ETS1 downwards and ETS1 participated in the regulation of the process CONCLUSIONS: ETS1 promotes proliferation, migration and invasion of lung adenocarcinoma cells by targeting the regulated miR-222 downwards.
肺腺癌是肺癌的一种类型。本研究旨在探讨miR-222对肺腺癌细胞增殖、迁移和侵袭的影响。
首先，将miR-222及其对照转染至肺腺癌细胞系A549，进行CCK-8增殖、Transwell迁移和基质胶侵袭实验，然后观察miR-222对肺腺癌细胞增殖、迁移和侵袭的影响。通过荧光素酶报告基因检测、实时荧光定量聚合酶链反应（RT-qPCR）和蛋白质免疫印迹法，下调ETS1对miR-222靶点进行调控，以参与该过程的调节。
根据CCK-8增殖实验、Transwell迁移实验和基质胶侵袭实验，发现miR-222可促进肺腺癌细胞的增殖、迁移和侵袭。荧光素酶报告基因检测、RT-qPCR和蛋白质免疫印迹实验表明，miR-222可下调ETS1的表达，且ETS1参与该过程的调节。结论：ETS1通过靶向下调miR-222促进肺腺癌细胞的增殖、迁移和侵袭。
