Dupuis A, Vignais P V
Biochem Biophys Res Commun. 1985 Jun 28;129(3):819-25. doi: 10.1016/0006-291x(85)91965-5.
An azido derivative of the oligomycin sensitivity conferring protein (OSCP) was prepared by alkylation with the bifunctional reagent p-azido phenacyl bromide. Azido-OSCP was fully biologically active in the dark. Upon photoirradiation of a mixture of beef heart mitochondrial F1-ATPase and azido-OSCP, the resulting covalent photoproducts were separated by polyacrylamide gel electrophoresis in the presence of Na dodecyl sulfate and characterized by an immunochemical procedure. OSCP was found to react with the alpha and the beta subunits of F1 with strong preference for the alpha subunit.
通过用双功能试剂对叠氮苯甲酰溴进行烷基化反应，制备了寡霉素敏感性赋予蛋白（OSCP）的叠氮衍生物。叠氮 - OSCP在黑暗中具有完全的生物活性。对牛心线粒体F1 - ATP酶和叠氮 - OSCP的混合物进行光照射后，在十二烷基硫酸钠存在下，通过聚丙烯酰胺凝胶电泳分离得到的共价光产物，并通过免疫化学方法进行表征。发现OSCP与F1的α和β亚基发生反应，对α亚基有强烈的偏好。
