Luo Honglin, Zhang Yang, Li Gen, Tu Junpin, Yang Zhiwei, Xiong Guangyao, Wang Zheren, Huang Yuan, Wan Yizao
1 School of Materials Science and Engineering, East China Jiao Tong University, Nanchang, Jiangxi, China.
2 School of Materials Science and Engineering, Tianjin University, Tianjn, None Selected, China.
J Biomater Appl. 2017 Aug;32(2):265-275. doi: 10.1177/0885328217715784. Epub 2017 Jun 15.
Three-dimensional nanofibrous scaffolds that morphologically mimic natural extracellular matrices hold great promises in tissue engineering and regenerative medicine due to their increased cell attachment and differentiation compared with block structure. In this work, for the first time, three-dimensional porous nanofibrous 58S bioglass scaffolds have been fabricated using a sacrificial template method. During the process, a natural three-dimensional nanofibrous bacterial cellulose was used as the sacrificial template on which precursor 58S glass was deposited via a sol-gel route. SEM and TEM results verify that the as-prepared 58S scaffolds can inherit the three-dimensional nanofibrous feature of bacterial cellulose. Pore structure characterizations by nitrogen adsorption-desorption and mercury intrusion porosimetry demonstrate that the 58S scaffolds are highly porous with a porosity of 75.1% and contain both mesopores (39.4 nm) and macropores (60 µm) as well as large BET surface area (127.4 m g). In vitro cell studies suggest that the 58S scaffold is bioactive and biocompatible with primary mouse osteoblast cells, suggesting that the nanofibrous structure of 58S is able to provide an appropriate environment for cellular functioning. These results strongly suggest that the three-dimensional nanofibrous 58S scaffold has great potential for application in bone tissue engineering and regenerative medicine.
三维纳米纤维支架在形态上模拟天然细胞外基质，与块状结构相比，其细胞附着和分化能力增强，因此在组织工程和再生医学领域具有广阔前景。在本研究中，首次采用牺牲模板法制备了三维多孔纳米纤维58S生物玻璃支架。在此过程中，天然三维纳米纤维细菌纤维素被用作牺牲模板，通过溶胶-凝胶法在其上沉积58S玻璃前驱体。扫描电子显微镜（SEM）和透射电子显微镜（TEM）结果证实，所制备的58S支架能够继承细菌纤维素的三维纳米纤维特征。通过氮气吸附-脱附和压汞法进行的孔隙结构表征表明，58S支架具有高度多孔性，孔隙率为75.1%，同时含有中孔（39.4nm）和大孔（60μm）以及较大的比表面积（127.4m²/g）。体外细胞研究表明，58S支架具有生物活性，并且与原代小鼠成骨细胞具有生物相容性，这表明58S的纳米纤维结构能够为细胞功能提供适宜的环境。这些结果有力地表明，三维纳米纤维58S支架在骨组织工程和再生医学领域具有巨大的应用潜力。
