School of Materials Science and Engineering, East China Jiaotong University, Nanchang 330013, China; School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China.
School of Materials Science and Engineering, East China Jiaotong University, Nanchang 330013, China.
Mater Sci Eng C Mater Biol Appl. 2017 Jul 1;76:94-101. doi: 10.1016/j.msec.2017.02.134. Epub 2017 Feb 28.
Three-dimensional (3D) nanofibrous scaffolds hold great promises in tissue engineering and regenerative medicine. In this work, for the first time, 3D SiO-CaO binary glass nanofibrous scaffolds have been fabricated via a combined method of template-assisted sol-gel and calcination by using bacterial cellulose as the template. SEM with EDS, TEM, and AFM confirm that the molar ratio of Ca to Si and fiber diameter of the resultant SiO-CaO nanofibers can be controlled by immersion time in the solution of tetraethyl orthosilicate and ethanol. The optimal immersion time was 6h which produced the SiO-CaO binary glass containing 60at.% Si and 40at.% Ca (named 60S40C). The fiber diameter of 60S40C scaffold is as small as 29nm. In addition, the scaffold has highly porous 3D nanostructure with dominant mesopores at 10.6nm and macropores at 20μm as well as a large BET surface area (240.9mg), which endow the 60S40C scaffold excellent biocompatibility and high ALP activity as revealed by cell studies using osteoblast cells. These results suggest that the 60S40C scaffold has great potential in bone tissue regeneration.
三维（3D）纳米纤维支架在组织工程和再生医学中有很大的应用前景。在这项工作中，首次通过模板辅助溶胶-凝胶和煅烧相结合的方法，以细菌纤维素为模板，制备了 3D SiO-CaO 二元玻璃纳米纤维支架。SEM 与 EDS、TEM 和 AFM 证实，通过在正硅酸乙酯和乙醇溶液中浸泡的时间，可以控制所得 SiO-CaO 纳米纤维的 Ca 与 Si 的摩尔比和纤维直径。最佳浸泡时间为 6h，制得的 SiO-CaO 二元玻璃中 Si 的含量为 60at.%，Ca 的含量为 40at.%（命名为 60S40C）。60S40C 支架的纤维直径小至 29nm。此外，该支架具有高度多孔的 3D 纳米结构，主要介孔为 10.6nm，大孔为 20μm，BET 比表面积大（240.9mg），这些特性使 60S40C 支架具有良好的生物相容性和较高的碱性磷酸酶（ALP）活性，这通过成骨细胞的细胞研究得到了证实。这些结果表明，60S40C 支架在骨组织再生方面具有很大的潜力。
